Research progress and prospects of nucleic acid isothermal amplification technology  

作　　者：SHUHUI WU PING XU XIANGBIN XU SONG-BAI LIU 

机构地区：[1]Suzhou Key Laboratory of Medical Biotechnology,Suzhou Vocational Health College,Suzhou,215009,China [2]School of Food Science and Engineering,Hainan University,Haikou,570228,China [3]The Affiliated Infectious Diseases Hospital,Suzhou Medical College of Soochow University,Suzhou,215100,China

出　　处：《BIOCELL》2023年第11期2385-2395,共11页生物细胞（英文）

基　　金：supported by grants from Jiangsu Higher Education Institution Innovative Research Team for Science and Technology(2021),the Key Technology Program of Suzhou People’s Livelihood Technology Projects(Grant Nos.SKY2021029,SZS2020311);the Open Project of Jiangsu Biobank of Clinical Resources(TC2021B009);the Qing-Lan Project of Jiangsu Province in China(2021,2022).

摘　　要：Nucleic acid(DNA and RNA)detection and quantification methods play vital roles in molecular biology.With the development of molecular biology,isothermal amplification of DNA/RNA,as a new molecular biology technology,can be amplified under isothermal condition,it has the advantages of high sensitivity,high specificity,and high efficiency,and has been applied in various fields of biotechnology,including disease diagnosis,pathogen detection,food hygiene and safety detection and so on.This paper introduces the progress of isothermal amplification technology,including rolling circle amplification(RCA),nucleic acid sequence-dependent amplification(NASBA),strand displacement amplification(SDA),loop-mediated isothermal amplification(LAMP),helicase-dependent amplification(HDA),recombinase polymerase amplification(RPA),cross-priming amplification(CPA),and its principle,advantages and disadvantages,and application development are briefly summarized.

关 键 词：Isothermal amplification Rolling circle amplification Nucleic acid sequence-based amplification Strand displacement amplification Loop-mediated isothermal amplification Helicase-dependent amplification Recombinase polymerase amplification Cross-primer amplification 

分 类 号：Q-4[生物学]