1株云南楚雄野生香菇菌株的分离鉴定及袋料栽培方法研究  

Isolation and Identification of a Wild Lentinula edodes Strain in Chuxiong,Yunnan and Bag Cultivation

作　　者：寸孟人 余浪 李蕾 王琴 陈玉惠 冯小飞 Cun Mengren;Yu Lang;Li Lei;Wang Qin;Chen Yuhui;Feng Xiaofei(College of Life Sciences,Southwest Forestry University,Kunming Yunnan 650233,China)

机构地区：[1]西南林业大学生命科学学院,云南昆明650233

出　　处：《西南林业大学学报（自然科学）》2023年第6期192-198,共7页Journal of Southwest Forestry University：Natural Sciences

基　　金：国家级大学生创新创业训练计划项目(202110677014)资助;云南省大学生创新创业训练计划项目(202110677007,202010677019)资助;生物学质量工程项目(503190106)资助;云南省教育厅科学研究基金项目(2022J0506)资助。

摘　　要：以云南楚雄地区的1株野生香菇为研究对象,开展了菌种的分离与鉴定,测定了不同温度、pH、碳源、氮源、无机盐对菌株菌丝体生长情况的影响;同时以杂木屑、玉米芯等农废资源为主要基质,进行了野生香菇菌株的袋料驯化栽培。结果表明:结合形态和ITS分子鉴定,菌株鉴定为香菇属真菌;生物学特性实验发现该野生香菇菌丝体生长最适温度为30℃,最适pH值为6.0,最佳碳源、氮源分别为玉米粉、蛋白胨,最适无机盐为氯化钙与硫酸镁;以60.0%杂木屑添加30.0%玉米芯组成的袋料基质栽培效果最好,生物转化率达到了41.49%。In the research,a wild Lentinula edodes strain from Yunnan Chuxiong area was isolated and characterized.Effect of different temperature,pH,carbon source,nitrogen source and inorganic salt on mycelia growth of the strain were studied.At the same time,the wild L.edodes strain was successfully cultivated by the mixed sawdust,corn cob and other agricultural waste resources as the main substrate.The results were as follows:Combined with morphological and ITS molecular identification,the strain was identified as Lentinula fungus by morphological and molecular identification;biological characteristics experiments found that the optimum temperature for the growth of wild L.edodes mycelium was 30.0℃,and the optimum pH was 6.0,the best carbon source and nitrogen source are corn meal and peptone,the best inorganic salt are calcium chloride and magnesium sulfate;bag substitute material cultivation results show:the optimal bag substitute composed of 60.0%sawdust and 30.0%corncob,the biotransformation rate reached 41.49%.

关 键 词：野生香菇 菌种分离 菌种鉴定 生物学特性 袋料栽培 

分 类 号：S318[农业科学—作物栽培与耕作技术]