日粮钙磷水平对马岗鹅生长性能及肝脏基因表达的影响  

Effect of dietary calcium and phosphorus level on Magang geese growth performance and liver gene expression

作　　者：李虹仪 周润盛 梁笑玲 张楚玥 吕祺欣 杨长华 张茂 LI Hongyi;ZHOU Runsheng;LIANG Xiaoling;ZHANG Chuyue;LYU Qixin;YANG Changhua;ZHANG Mao(Henry Fok College of Biology and Agriculture,Shaoguan University,Shaoguan 512000,Guangdong,China)

机构地区：[1]韶关学院英东生物与农业学院,广东韶关512000

出　　处：《浙江农业学报》2023年第11期2533-2542,共10页Acta Agriculturae Zhejiangensis

基　　金：广东省大学生创新创业训练计划(S202110576068);广东省自然科学基金面上项目(2021A1515010483)。

摘　　要：为了研究日粮中钙磷水平对马岗鹅生长性能及肝脏代谢相关基因的影响。试验选用48只体重相近、公母各半的50日龄马岗鹅,随机分为3个组,每组2个重复,每个重复8只,每笼饲养2只。3个组在相同日粮(含钙0.16%、有效磷0.11%)的基础上,分别添加0%、0.15%和0.3%的磷酸氢钙。预饲5 d,试验期28 d,期间记录日采食量,定期采集粪样及血样,测定样品中的钙磷含量。试验结束每组随机抽取8只马岗鹅进行屠宰及肝脏组织样采集,测定其屠宰性能和磷酸化酶活性,转录组测序及荧光定量PCR检测肝脏基因的表达变化。结果表明:对照组试验鹅的采食量高于其他两组(P<0.05),平均日增重、屠宰性能均高于0.15%磷酸氢钙组(P<0.05),而与0.3%磷酸氢钙组无显著差异;3组鹅血钙血磷含量无显著性差异(P>0.05),而粪钙含量随钙磷水平的提高而增加(P<0.05)。转录组测序结果显示,对照组与0.15%磷酸氢钙组之间差异较少,而与0.3%磷酸氢钙组差异较明显,经过分析,差异显著基因较多富集在糖代谢通路,荧光定量PCR结果亦与转录组测序一致,糖代谢相关酶磷酸化酶的活性则随钙磷水平的提高而减少(P<0.05)。日粮中钙磷水平会影响马岗鹅的采食量,同时影响肝脏的糖脂代谢。To study the effect of calcium and phosphorus levels in diet on growth performance and liver metabolism related genes in Magang geese,a total of forty eight Magang geese of 50-day-old were randomly divided into three groups,with male and female in half.Each group had two replicates with eight geese per replicate,every two geese were kept in one cage.On the basis of the same diet,0%,0.15%and 0.3%calcium hydrogen phosphate were added respectively,each group was pre-fed for five days and fed for twenty-eight days,during which the daily feed intake was recorded,fecal samples and blood samples were collected regularly,and the content of calcium and phosphorus in geese samples were determined.At the end of the experiment,eight Magang geese were randomly selected from each group for slaughter and the slaughter performance and phosphorylase activity were measured.The liver tissue of each geese were collected for transcriptome sequencing,fluorescence quantitative PCR and enzyme activity detection.The results showed that the feed intake of control group was significantly higher than that of the other two groups(P<0.05).The average daily gain and carcass traits were significantly higher than that of 0.15%level group,with no significant difference from 0.3%level group(P>0.05).In addition,there was no significant difference in the content of calcium and phosphorus in blood among the three groups(P>0.05),but the content of fecal calcium increased with the increase of calcium and phosphorus level(P<0.05).Transcriptome sequencing results showed that there was less difference between control group and 0.15%level group,but more significant difference between control group and 0.3%level group.And most of the differently expressed genes were enriched in sugar metabolism pathway,which was consistent with the quantitative PCR results.Also the activity of phosphorylases,the glucose metabolism-related enzyme,decreased with the increase of calcium and phosphorus levels(P<0.05).In a word,the level of calcium and phosphorus in the diet wil

关 键 词：马岗鹅 钙磷水平 生长性能 基因表达 代谢 

分 类 号：S835[农业科学—畜牧学]