云南山茶在干旱-复水过程中抗氧化酶活性变化及关键基因差异表达分析  

Changes of antioxidant enzyme activity and differential expression of key genes in Camellia reticulata during drought-rehydration process

作　　者：林先玉 李紫倩 柏松 罗军 屈燕[1,2] LIN Xianyu;LI Ziqian;BAI Song;LUO Jun;QU Yan(School of Landscape Architecture,Southwest Forestry University,Kunming 650224,China;Southwest Landscape Architecture Engineering Technology Research Center,National Forestry and Grassland Administration,Kunming 650224,China)

机构地区：[1]西南林业大学园林园艺学院,云南昆明650224 [2]国家林业和草原局西南风景园林工程技术研究中心,云南昆明650224

出　　处：《浙江农业学报》2023年第11期2611-2620,共10页Acta Agriculturae Zhejiangensis

基　　金：“十三五”国家重点研发计划(2019YFD1001005);云南省万人计划青年拔尖人才项目(YNWR-QNBJ-2019-211)。

摘　　要：为研究在干旱胁迫及复水过程中云南山茶抗氧化酶活性变化及关键基因的表达差异,本文以半年生云南山茶幼苗为材料,利用聚乙二醇(PEG-6000)对其进行干旱胁迫,测定云南山茶叶片过氧化物酶(POD)和超氧化物歧化酶(SOD)活性,并将对照组和重度干旱组的叶片进行转录组测序。结果表明:在胁迫期间,各处理组SOD活性持续上升,重度干旱胁迫下POD活性先上升后下降;在复水期间,POD、SOD活性都呈下降趋势,除轻度干旱胁迫组外其余两组POD和SOD活性均未能恢复到胁迫之前的水平。通过对关键基因差异表达分析发现,在重度干旱胁迫及复水过程中,33个抗氧化酶相关基因表达量与抗氧化酶活性有极显著相关性(P<0.01),19个显著相关(P<0.05)。POD相关基因均富集于苯丙烷类生物合成通路中,SOD相关基因主要富集于过氧化物酶体通路中,均为下调表达。在胁迫开始后相关基因表达量均发生了显著性变化,说明抗氧化酶相关基因在干旱胁迫及复水过程中参与了调控,积极响应干旱胁迫。To study the changes of antioxidant enzyme activity and the expression of key genes in Camellia reticulata during drought stress and rehydration process,this paper used semi-annual Camellia reticulata seedlings to determine the peroxidase(POD)and superoxide dismutase(SOD)activities of Camellia reticulata leaves under drought stress using polyethylene glycol(PEG-6000),and transcriptome sequencing was performed on leaves of the control group and the severely drought stress group.The results showed that during the stress period,the SOD activity of each treatment group showed a continuous upward trend,and the POD activity firstly increased and then decreased under severe drought stress.During the rehydration period,the activities of POD and SOD showed a downward trend,except for the mild drought stress group,the POD and SOD activities of the other two groups could not recover to the level before stress.Through the analysis of differential expression of key genes,it was found that the expression of 33 antioxidant enzyme-related genes was very significantly correlated with antioxidant enzyme activity(P<0.01)and 19 antioxidant enzyme-related genes showed significant correlation(P<0.05)during severe drought stress and rehydration.POD-related genes were enriched in the phenylpropane biosynthesis pathway,and SOD-related genes were mainly enriched in the peroxisome pathway,all of which were down-regulated.The relevant expression levels changed significantly after the start of stress,indicating that antioxidant enzyme-related genes participated in the regulation of drought stress and rehydration,and actively responded to drought stress.

关 键 词：云南山茶 干旱胁迫 复水 抗氧化酶 基因表达 

分 类 号：S685.14[农业科学—观赏园艺]