Zinc oxide nanoparticles accelerate the healing of methicillin-resistant Staphylococcus aureus(MRSA)-infected wounds in rabbits  

作　　者：Muhammad Asif Ayesha Safdar Chaudhry Ambreen Ashar Hamad Bin Rashid Muhammad Hassan Saleem Hassaan Bin Aslam Abdul Aziz 

机构地区：[1]Department of Veterinary Surgery,University of Veterinary and Animal Sciences,Lahore,Punjab,Pakistan [2]TECS Department,Wilson College of Textiles,NC State University,Raleigh 27606,USA [3]Department of Veterinary Medicine,University of Veterinary and Animal Sciences,Lahore,Punjab,Pakistan [4]Institute of Microbiology,University of Veterinary and Animal Sciences,Lahore,Punjab,Pakistan [5]Department of Veterinary Clinical Sciences,University of Poonch,Rawalakot 12350,Pakistan

出　　处：《Asian Pacific Journal of Tropical Biomedicine》2023年第11期488-496,I0002,共10页亚太热带生物医学杂志（英文版）

摘　　要：Objective:To synthesize zinc oxide nanoparticles(ZnONPs)and evaluate their antibacterial and wound healing effects against wounds infected with methicillin-resistant Staphylococcus aureus(MRSA).Methods:ZnONPs were prepared by sol-gel method and characterized by X-ray diffraction(XRD)analysis and scanning electron microscopy(SEM).A total of 18 rabbits were divided into three groups:the ZnONPs group,the gentamicin group and the control group.A wound of 3 cm^(2) was inflicted on each rabbit and contaminated with MRSA inoculum.Treatment was started from the fourth day post-surgery.Wound healing,microbiological analysis,and histopathological analysis were performed to assess the efficacy of ZnONPs ointment.Results:XRD analysis confirmed the hexagonal wurtzite structure of the ZnONPs with an average crystallite size of 29.23 nm.SEM revealed discoid-shaped ZnONPs with a rough surface and an average size of 48.36 nm.Energy-dispersive X-ray analysis confirmed the purity of ZnONPs.Moreover,the particle size ranged from 100-700 nm with a high agglomeration trend.Treatment with ZnONPs promoted MRSA-infected wound healing.In addition,ZnONPs showed a good antibacterial effect as evidenced by a dose-dependent increase in the zone of inhibition.Conclusions:ZnONPs accelerate the healing of MRSA-infected wounds.Therefore,it can be explored for the treatment of MRSA infection.

关 键 词：MRSA Wound healing ZnO NANOPARTICLES OINTMENT 

分 类 号：R378[医药卫生—病原生物学]