广东省稻菜轮作区中牛筋草对十种除草剂的抗性水平及靶基因序列分析  

Analyses of the resistance level and target enzyme gene sequence of Indian goosegrass Eleusine indica to ten herbicides in rice and vegetable rotation fields of Guangdong Province

作　　者：张纯[1] 冯凯帆 郭文磊 张泰劼[1] 田兴山[1] Zhang Chun;Feng Kaifan;Guo Wenlei;Zhang Taijie;Tian Xingshan(Guangdong Provincial Key Laboratory of High Technology for Plant Protection,Plant Protection Research Institute,Guangdong Academy of Agricultural Sciences,Guangzhou 510640,Guangdong Province,China)

机构地区：[1]广东省农业科学院植物保护研究所,广东省植物保护新技术重点实验室,广州510640

出　　处：《植物保护学报》2023年第5期1210-1218,共9页Journal of Plant Protection

基　　金：国家自然科学基金(31972282,32272568);广东省科技计划(2019B121201003);广东省农业科学院学科团队及人才培育项目(202105TD,R2020PY-JX005)。

摘　　要：为明确广东省稻菜轮作区中牛筋草对10种常用除草剂的抗性水平及抗性分子机制,采用整株生物测定法测定广东省稻菜轮作区内8个牛筋草种群P1~P8对草甘膦、草铵膦和乙酰辅酶A羧化酶(acetyl-CoA carboxylase,ACCase)抑制剂类等10种除草剂的抗性水平,并进一步分析P1和P8种群相关靶标酶基因5-烯醇丙酮酰莽草酸-3-磷酸合酶(5-enolpyruvyl-shikimate-3-phosphate synthase,EPSPS)、谷氨酰胺合成酶(glutamine synthetase,GS)和ACCase的部分功能区序列特征。结果显示,牛筋草P1~P8种群对草甘膦抗性指数为敏感种群的5.9倍~17.7倍,其中P8种群对草甘膦的抗性水平最高;8个种群对草铵膦也产生了不同程度的抗性,抗性指数为敏感种群的2.3倍~14.2倍,其中P1种群抗性最高。牛筋草P1和P8种群均对ACCase抑制剂类除草剂精喹禾灵、氰氟草酯和噁唑酰草胺产生了交互抗性;P1种群ACCase基因在第2041位氨基酸处发生突变,该突变在牛筋草种群中首次发现;而P8种群ACCase基因则在第2027位氨基酸处发生突变。牛筋草高抗种群P1和P8及敏感种群对乙酰乳酸合成酶(acetolactate synthase,ALS)、对羟基苯基丙酮酸双加氧酶(4-hy-droxyphenylpyruvate dioxygenase,HPPD)抑制剂类除草剂以及合成激素类除草剂均不敏感。尽管ACCase抑制剂类除草剂烯草酮尚能有效防控部分牛筋草种群,但从长远来看,有必要加强杂草绿色综合防控技术研究与应用,以延缓抗性杂草种群的进化和蔓延。In order to determine the resistance level and mechanism of target-gene molecular resistance of Indian goosegrass Eleusine indica in rice and vegetable rotation fields to ten kinds of commonly used herbicides,such as glyphosate,glyphosate,acetyl CoA carboxylase(ACCase)inhibitor herbicides,acetolactate synthase(ALS)inhibitor herbicides,4-hy-droxyphenylpyruvate dioxygenase(HPPD)inhibitor herbicides and quinclorac,the whole-plant bioassay was used to test the resistance level of eight E.indica populations(P1-P8)collected in the farmlands of Guangdong Province,as well as the target enzyme gene sequences of the functional regions of 5-enolpyruvyl-shikimate-3-phosphate synthase(EPSPS),glutamine synthase(GS),and ACCase were detected.It showed that the populations P1-P8 were highly resistant to glyphosate and glufosinate.Dose-response experiments with glyphosate showed that the eight populations are 5.9-17.7 fold resistant compared with the susceptible population,P8 showed the highest resistance to glyphosate.The eight populations also displayed resistance to glufosinate,the resistance index is between 2.3 and 14.2,and P1 has evolved high resistance to glufosinate.In addition,highly resistant populations of E.indica P1 and P8 also displayed cross resistance to ACCaseinhibiting herbicides including quizalofop-P-ethyl,cyhalofop-butyl and metamifop,but the molecular mechanisms are different.ACCase gene sequencing revealed that the mutation at amino acid 2041 in P1 and 2027 in P8 are likely responsible for resistance to the ACCase inhibitors examined.The populations P1 and P8 were insensitive to ALS-inhibiting herbicides,HPPD-inhibiting herbicide and synthetic auxin herbicides.Although clethodim could provide effective control,in the long run,additional diversity in weed control must be added,to delay resistance evolution and spreading of E.indica population.

关 键 词：牛筋草 除草剂 ACCase抑制剂类除草剂 抗性水平 交互抗性 

分 类 号：S451[农业科学—植物保护]