电针对脑缺血再灌注大鼠内质网应激IRE1α/TRAF2/ASK1/Caspase-12通路的影响研究  

Effect of EA on IRE1α/TRAF2/ASK1/Caspase-12 Pathway under Endoplasmic Reticulum Stress in CIRI Rats

作　　者：聂妍琦 傅旖灵 刘孜琦 陆梦 郁洁[1] 雷晓明[1] NIE Yanqi;FU Yiling;LIU Ziqi;LU Meng;YU Jie;LEI Xiaoming(Hunan University of Chinese Medicine,Changsha 410208,China)

机构地区：[1]湖南中医药大学,湖南长沙410208

出　　处：《针灸临床杂志》2023年第11期66-72,共7页Journal of Clinical Acupuncture and Moxibustion

基　　金：湖南省中医药科研计划项目重点课题,编号:湘中医药[2019]1号;湖南省教育厅优秀青年项目,编号:湘教通[2019]90号;湖南中医药大学中医学一级学科开放基金项目重点项目,编号:2018ZYX06;湖南中医药大学科研基金项目,编号:2018XJJJ01。

摘　　要：目的:观察电针“百会”“内关”对脑缺血再灌注损伤(CIRI)大鼠缺血侧大脑皮质凋亡相关蛋白IRE1α、TRAF2、ASK1及Caspase-12的影响,初步探讨电针治疗脑缺血再灌注凋亡损伤的作用机制。方法:60只雄性SD大鼠,随机分为假手术组(A组)、模型组(B组)、针刺组(C组)和依达拉奉组(D组),每组15只。选用改良线栓法建立大鼠大脑中动脉阻塞(MCAO)模型;LONGA 5级神经功能评分法评估大鼠神经功能情况;苏木素-伊红(HE)染色法观察大鼠缺血侧大脑皮质病理形态学变化;原位末端转移酶标记法(TUNEL)检测大鼠神经元细胞凋亡率;蛋白免疫印迹法(WB)检测大鼠大脑皮质IRE1α、TRAF2、ASK1及Caspase-12蛋白表达情况。结果:与假手术组比较,模型组大鼠神经功能评分,细胞凋亡率以及IRE1α、TRAF2、ASK1及Caspase-12蛋白表达量均明显增加,差异具有统计学意义(P<0.01),大脑皮质组织结构紊乱,细胞萎缩坏死;与模型组比较,针刺组和依达拉奉组神经功能缺损评分以及IRE1α、TRAF2、ASK1和Caspase-12蛋白表达量均明显降低,差异具有统计学意义(P<0.01),且细胞凋亡率降低,差异具有统计学意义(P<0.01或P<0.05),大脑皮质细胞病理状况比模型组减轻,萎缩坏死细胞变少;与依达拉奉组比较,针刺组大鼠各项指标差异无统计学意义(P>0.05)。结论:电针能抑制CIRI大鼠脑组织细胞凋亡,促进神经功能恢复,作用机制可能与电针下调IRE1α/TRAF2/ASK1/Caspase-12通路蛋白有关。Objective:To observe the effect of electro-needling Baihui(DU20)and Neiguan(PC6)on apoptosis related proteins of IRE1α,TRAF2,ASK1 and Caspase-12 in ischemic brain tissues of rats with cerebral ischemia-reperfusion injury(CIRI),thus to explore the specific mechanism of electro-acupuncture(EA)in treatment of CIRI.Methods:60 male SD rats were randomly divided into the sham operation group,the model group,the acupuncture group and the Edaravone group,with 15 rats in each group.The model of middle cerebral artery occlusion(MCAO)was established by using modified suture method.LONGA five-grade neurological function score was used to evaluate neurological function.HE staining was used to observe pathological changes of ischemic brain tissues.TUNEL method was used to detect apoptosis rate of neurons.The protein expressions of IRE1α,TRAF2,ASK1 and Caspase-12 in ischemic brain tissues were detected by Western blotting method.Results:Compared with those in the sham operation group,the the neurological deficit score,the apoptosis rate and the protein expressions of IRE1α,TRAF2,ASK1 and Caspase-12 were significantly increased(P<0.01);the cortex tissues were disorderly arranged with atrophic and necrotic cells in the model group.Compared with those in the model group,the neurological deficit score,the protein expressions of IRE1α,TRAF2,ASK1 and Caspase-12 were significantly decreased(P<0.01),and the apoptosis rate was also significantly decreased(P<0.01,P<0.05);the pathological condition of cortex was alleviated with less atrophic and necrotic cells in the acupuncture group and the Edaravone group.There were no statistical differences in the above indexes between the acupuncture group and the Edaravone group(P>0.05).Conclusion:EA can inhibit the apoptosis of brain tissues in CIRI rats,and promote the recovery of neural function,which may be related to down-regulating the proteins of IRE1α/TRAF2/ASK1/Caspase-12 pathway.

关 键 词：脑缺血再灌注 凋亡 IRE1α 电针 内质网应激通路 

分 类 号：R246.6[医药卫生—针灸推拿学]