A lipid droplet-associated protein Nem1 regulates appressorium function for infection of Magnaporthe oryzae  

作　　者：Deng Chen Xuan Cai Junjie Xing Shen Chen Juan Zhao Zhiguang Qu Guotian Li Hao Liu Lu Zheng Junbin Huang Xiao-Lin Chen 

机构地区：[1]State Key Laboratory of Agricultural Microbiology and Provincial Key Laboratory of Plant Pathology of Hubei Province,College of Plant Science and Technology,Huazhong Agricultural University,Wuhan 430070,China [2]State Key Laboratory of Hybrid Rice,Hunan Hybrid Rice Research Center,Changsha 410125,China [3]Guangdong Provincial Key Laboratory of High Technology for Plant Protection,Plant Protection Research Institute,Guangdong Academy of Agricultural Sciences,Guangzhou 510640,China

出　　处：《aBIOTECH》2023年第2期108-123,共16页生物技术通报（英文版）

基　　金：supported by the National Natural Science Foundation of China(Grants 32072365 and 32272476);the Open Research Fund of the State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China(SKL-KF202216).

摘　　要：Lipid droplets are important storages in fungal conidia and can be used by plant pathogenic fungi for infection.However,the regulatory mechanism of lipid droplets formation and the utilization during fungal development and infection are largely unknown.Here,in Magnaporthe oryzae,we identified a lipid droplet-associated protein Nem1 that played a key role in lipid droplets biogenesis and utilization.Nem1 was highly expressed in conidia,but lowly expressed in appressoria,and its encoded protein was localized to lipid droplets.Deletion of NEM1 resulted in reduced numbers of lipid droplets and decreased content of diacylglycerol(DAG)or triacylglycerol(TAG).NEM1 was required for asexual development especially conidia production.TheΔnem1 mutant was nearly loss of virulence to host plants due to defects in appressorial penetration and invasive growth.Remarkably,Nem1 was regulated by the TOR signaling pathway and involved in the autophagy process.The Ser303 residue of Nem1 could be phosphorylated by the cAMP-PKA signaling pathway and was important for biological function of Nem1.Together,our study revealed a regulatory mechanism of lipid biogenesis and metabolism during the conidium and appressorium formation of the rice blast fungus.

关 键 词：Lipid biogenesis TOR signaling cAMP-PKA signaling Conidium formation APPRESSORIUM Magnaporthe oryzae 

分 类 号：S435[农业科学—农业昆虫与害虫防治]