一株鸭源大肠杆菌噬菌体vB_EcoS214的分离鉴定  

Isolation and identification of a phage vB_EcoS214 of Escherichia coli from duck

作　　者：刘娜 陈红梅[1] 江南松 黄瑜[1] 傅光华[1] 程龙飞[1] Liu Na;Chen Hongmei;Jiang Nansong;Huang Yu;Fu Guanghua;Cheng Longfei(Fujian Animal Diseases Control Technology Center/Institute of Animal Husbandry and Veterinary Medicine,Fujian Academy of Agricultural Sciences/Fujian Key Laboratory for Avian Diseases Control and Prevention,Fuzhou350013)

机构地区：[1]福建省畜禽疫病防治工程技术研究中心/福建省农业科学院畜牧兽医研究所/福建省禽病防治重点实验室,福州350013

出　　处：《福建畜牧兽医》2023年第6期12-14,共3页Fujian Journal of Animal Husbandry and Veterinary medicine

基　　金：财政部和农业农村部:国家现代农业产业技术体系项目(CARS-42);农业科技创新联盟专项(CXLM202205);福建省农业高质量发展超越“5511”协同创新工程项目(XTCXGC2021012)资助。

摘　　要：为分离能裂解鸭源大肠杆菌的噬菌体,采用富集法从鸭场污水中分离噬菌体,透射电镜观察其形态,结果显示,分离到1株噬菌体,电镜显示该噬菌体属长尾病毒科,命名为vB_EcoS214,头部直径约40 nm,尾部长约220 nm,宽约10 nm。该噬菌体有较宽的裂解谱,裂解力较强,可成为噬菌体鸡尾酒制剂的候选噬菌体。能裂解鸭源大肠杆菌噬菌体的成功分离,为鸭源大肠杆菌病的噬菌体疗法提供参考。In order to isolate phages that can cleave Escherichia coli from duck,phages were isolated from duck farm sewage by enrichment method.Bacteriophage morphology and size were examined with a transmission electron microscope(TEM).The results showed that a phage was isolated.TEM showed that the phage belonged to the family Siphoviridae,named vB_EcoS214.The diameter of the head is about 40 nm,the length of the tail is about 220 nm and the width is about 10 nm.The phage has a wide cleavage spectrum and strong cleavage force,and it can be a candidate for cocktail preparation of phage.The successful isolation of phage that can cleave Escherichia coli from duck provide reference for phage therapy of duck colibacillosi.

关 键 词：鸭 大肠杆菌 噬菌体 

分 类 号：S852.61[农业科学—基础兽医学]