贵州山羊大肠杆菌的分离鉴定及药敏试验  

Isolation,Identification and Drug Sensitivity Test of Escherichia Coli from Guizhou Goat

作　　者：杨莉[1] 李婷 陈雪娇 徐景峨[1] 韩勇[1] 周伟 陶孟旭 李正兴 杨茂生[1] 李美娟 蒲龄 周国松 张涛[1] 田宇杰 卢昱希 周思旋[1] YANG Li;LI Ting;CHEN Xuejiao;XU Jing'e;HAN Yong;ZHOU Wei;TAO Mengxu;LI Zhengxing;YANG Maosheng;LI Meijuan;PU Ling;ZHOU Guosong;ZHANG Tao;TIAN Yujie;LU Yuxi;ZHOU Sixuan(Guizhou Institute of Animal Husbandry and Veterinary Medicine,Guiyang Guizhou 550005,China;Wudang Campus of Guizhou Normal University,Guiyang Guizhou 550006,China;Animal Disease Prevention and Control Center of Xingren County,Xingren Guizhou 561409,China;Animal Disease Prevention and Control Center of Nayong County,Nayong Guizhou 553399,China;Animal Disease Prevention and Control Center of Qinglong County,Qinglong Guizhou 561400,China)

机构地区：[1]贵州省畜牧兽医研究所,贵州贵阳550005 [2]贵州师范学院乌当校区,贵州贵阳550006 [3]贵州省兴仁县动物疫病预防控制中心,贵州兴仁561409 [4]贵州省纳雍县动物疫病预防控制中心,贵州纳雍553399 [5]贵州省晴隆县动物疫病预防控制中心,贵州晴隆561400

出　　处：《现代畜牧科技》2023年第11期10-13,共4页Modern Animal Husbandry Science & Technology

基　　金：毕节试验区梅花鹿及黑山羊特色产业提质增效配套技术集成与示范应用(黔科合服企[2020]4009);梅花鹿及黑山羊常见疫病防治示范应用(黔科合服企[2020]4009(003));羊原种场主要疫病净化技术研究(黔科合支撑[2022]一般140);贵州省科技支撑项目:羊新发病毒性疫病的诊断和防控技术研究(黔科合支撑[2023]一般084)。

摘　　要：为分离贵州山羊大肠杆菌和了解其耐药情况,通过对贵州贵阳、晴隆、纳雍、威宁和兴仁山羊场进行无菌采集肛门拭子、病死山羊的肝等347份样品,大肠杆菌的分离培养、24种生化试验反应、16S rDNA PCR鉴定和21种抗生素药敏试验等,分离、生化和16S rDNA PCR鉴定出264株山羊大肠杆菌,贵州每个山羊场对氨苄西林和头孢硫脒产生高度耐药,对复方新诺明、头孢西丁、头孢呋辛、头孢他啶和米诺环素耐药,其他抗生素药敏感,这为贵州省控制和预防山羊大肠杆菌病提供科学依据。In order to isolate Escherichia coli from Guizhou goat and understand its drug resistance,more than 347 samples including anal swabs and liver samples from diseased goat were collected aseptically from goat farms in Guiyang,Qinglong,Nayong,Weining,and Xingren,Guizhou.The isolation and cultivation of Escherichia coli,24 biochemical test reactions,16S rDNA PCR identification,and 21 antibiotic susceptibility tests were conducted Biochemical and 16S rDNA PCR identified 264 strains of Escherichia coli from goat.Each goat farm in Guizhou developed high resistance to ampicillin and cefotaxime,followed by resistance to compound sulfamethoxazole,cefoxitin,cefuroxime,ceftazidime,and minocycline.Other antibiotic drugs were sensitive,providing scientific basis for the control and prevention of Escherichia coli disease in goat in our province.

关 键 词：山羊 大肠杆菌 PCR 耐药性 

分 类 号：S852.61[农业科学—基础兽医学]