电针对代谢综合征大鼠糖脂代谢和IRS-1/PI3K/Akt/GLUT4信号通路的影响  

Effects of Electro-acupuncture on Glucolipid Metabolism and IRS-1/PI3K/Akt/GLUT4 Signaling Pathway in Rats with Metabolic Syndrome

作　　者：黄碗文 林智摸 余焯燊[3] 米建平[3] HUANG Wan-Wen;LIN Zhi-Mo;YU Zhuo-Shen;MI Jian-Ping(The Second Clinical Medical School,Guangzhou University of Chinese Medicine,Guangzhou 510405 Guangdong,China;Dept.of Pediatric Rehabilitation,Shenzhen Luohu Hospital of Traditional Chinese Medicine,Shenzhen 518009 Guangdong,China;Dept.of Traditional Therapies,Guangdong Provincial Hospital of Traditional Chinese Medicine,Guangzhou 510102 Guangdong,China)

机构地区：[1]广州中医药大学第二临床医学院,广东广州510405 [2]深圳市罗湖中医院儿童康复科,广东深圳518009 [3]广东省中医院传统疗法科,广东广州510102

出　　处：《广州中医药大学学报》2023年第11期2839-2846,共8页Journal of Guangzhou University of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(编号:82074342)。

摘　　要：【目的】探讨电针对代谢综合征(MS)大鼠糖脂代谢和胰岛素受体底物1(IRS-1)/磷脂酰肌醇-3-激酶(PI3K)/蛋白激酶B(Akt)/葡萄糖转运蛋白4(GLUT4)信号通路的影响。【方法】采用高脂高糖高盐饲料喂养方法构建代谢综合征大鼠模型,将造模成功的大鼠随机分为模型组、二甲双胍组和电针组,另设正常组。二甲双胍组大鼠给予盐酸二甲双胍片的生理盐水混悬液灌胃,电针组大鼠给予关元、中脘、足三里、丰隆穴电针治疗,正常组和模型组大鼠只固定,不针刺。连续干预21 d后,测量大鼠体质量、腹腔脂肪质量和血压,计算Lee’s指数和体脂比,检测空腹血糖(FPG)、空腹胰岛素(FINS)、血脂、脂肪酸(FFA)、脂联素(APN),计算胰岛素抵抗指数(HOMA-IR);分别采用实时定量聚合酶链反应(qRT-PCR)和Western Blot法检测肝脏组织IRS-1、PI3K、Akt、GLUT4的mRNA和蛋白表达;苏木素-伊红(HE)染色法观察肝脏和胸主动脉病理变化。【结果】与正常组比较,模型组大鼠体质量、Lee’s指数、腹腔脂肪质量、体脂比、收缩压(SBP)、舒张压(DBP)、FPG、FINS、HOMA-IR、总胆固醇(TG)、甘油三酯(TC)、低密度脂蛋白胆固醇(LDL-C)、FFA水平升高(P<0.05),高密度脂蛋白胆固醇(HDL-C)、APN水平降低(P<0.05),肝脏IRS-1、PI3K、Akt、GLUT4 mRNA和蛋白表达水平降低(P<0.05),HE染色结果显示肝脏脂肪变性和胸主动脉粥样硬化。与模型组比较,电针组和二甲双胍组大鼠体质量、Lee’s指数、腹腔脂肪质量、体脂比、SBP、DBP、FPG、FINS、HOMA-IR、TG、TC、LDL-C、FFA水平降低(P<0.05),HDL-C、APN水平升高(P<0.05),肝脏IRS-1、PI3K、Akt、GLUT4 mRNA和蛋白表达水平升高(P<0.05),HE染色结果显示肝脏脂肪变性和胸主动脉粥样硬化明显减轻。电针组和二甲双胍组上述指标改变差异无统计学意义(P>0.05)。【结论】电针可以改善代谢综合征大鼠的糖脂代谢,其机制可能与上调IRS-1/PI3K/Akt/Objective To investigate the effects of electro-acupuncture on glucolipid metabolism and insulin receptor substrate 1(IRS-1)/phosphatidylinositol-3-kinase(PI3K)/protein kinase B(Akt)/glucose transporter protein 4(GLUT4)signaling pathway in rats with metabolic syndrome(MS).Methods The MS rat model was constructed by feeding with high-fat,high-sugar and high-salt feeds,and the successful rats were randomly divided into the model group,the metformin group and the electro-acupuncture group,and the normal group was also set up.Rats in the metformin group were given saline suspension of Metformin Hydrochloride Tablets by gavage,and rats in the electro-acupuncture group were given electro-acupuncture treatment at Guanyuan(RN4),Zhongwan(RN12),Zusanli(ST36),and Fenglong(ST40)points,and rats in the normal group and the model group were fixed only without acupuncture.At the end of the intervention for 21 consecutive days,body mass,abdominal fat mass and blood pressure were detected,Lee’s index and body fat ratio were calculated,fasting plasma glucose(FPG),fasting insulin(FINS),blood lipids,fatty acids(FFA),and adiponectin(APN)were measured,and insulin resistance index(HOMA-IR)was calculated;quantitative real-time polymerase chain reaction(qRT-PCR)and Western Blot were used to test the mRNA and protein expressions of IRS-1,PI3K,Akt,and GLUT4;hematoxylin-eosin(HE)staining was used to observe pathological changes in the liver and thoracic aorta.Results Compared with the normal group,the body mass,Lee’s index,abdominal fat mass,body fat ratio,systolic blood pressure(SBP),diastolic blood pressure(DBP),FPG,FINS,HOMA-IR,total cholesterol(TG),triglyceride(TC),low-density lipoprotein cholesterol(LDL-C)and FFA levels were increased in the rats of the model group(P<0.05),HDL-C and APN levels were decreased(P<0.05),mRNA and protein expressions of hepatic IRS-1,PI3K,Akt,GLUT4 were decreased(P<0.05),and HE staining results showed hepatic steatosis and thoracic aortic artery atherosclerosis.Compared with the model group,the body mass,

关 键 词：电针 代谢综合征 糖脂代谢 胰岛素抵抗 IRS-1/PI3K/Akt/GLUT4信号通路 大鼠 

分 类 号：R245[医药卫生—针灸推拿学]