浆细胞性乳腺炎血清长链非编码RNA牛磺酸上调基因1与微小RNA-142-5p表达临床意义  

Clinical significance of serumLncRNA TUG1and miR-142-5p expression in plasma cell mastiti

作　　者：石长勇 周紫珍 熊益敏 周广林 SHI Changyong;ZHOU Zizhen;XIONG Yimin;ZHOU Guanglin(Longgang District Maternity&Child Healthcare Hospital of Shenzhen City(Longgang Maternity and Child Institute of Shantou University Medical College),Shenzhen,Guangdong518172,China)

机构地区：[1]深圳市龙岗区妇幼保健院(汕头大学医学院龙岗妇幼临床学院)乳腺外科,广东深圳518172 [2]深圳市龙岗区妇幼保健院(汕头大学医学院龙岗妇幼临床学院)影像科,广东深圳518172

出　　处：《社区医学杂志》2023年第21期1130-1135,共6页Journal Of Community Medicine

摘　　要：目的探讨血清长链非编码RNA牛磺酸上调基因1(LncRNA TUG1)、微小RNA-142-5p(miR-142-5p)检测水平对浆细胞性乳腺炎复发的预测价值。方法选取2019-01-01-2022-10-01深圳市龙岗区妇幼保健院收治的浆细胞性乳腺炎患者211例为研究对象,收集其临床特征,根据患者恢复正常1个月后是否复发分为复发组43例和未复发组168例。采用实时荧光定量PCR(qRT-PCR)法检测血清LncRNA TUG1、miR-142-5p水平,数据采用2-ΔΔCt法计算;采用受试者工作特征(ROC)曲线分析血清LncRNA TUG1、miR-142-5p水平对浆细胞性乳腺炎复发的预测价值;采用logistic回归分析检验浆细胞性乳腺炎复发的影响因素。结果乳头状态凹陷的浆细胞性乳腺炎患者血清LncRNA TUG1水平为0.82±0.16,低于正常患者1.32±0.21,差异有统计学意义,t=18.594,P<0.05。miR-142-5p水平为1.13±0.27,高于正常患者1.01±0.20,差异有统计学意义,t=3.098,P<0.05;分期为急性期的浆细胞性乳腺炎患者血清LncRNA TUG1水平为0.66±0.15,低于亚急性期0.96±0.21和慢性期患者1.11±0.22,差异有统计学意义,F=64.774,P<0.05。miR-142-5p水平为1.37±0.28,高于亚急性期1.11±0.27和慢性期患者0.94±0.20,差异有统计学意义,F=40.428,P<0.05。复发组血清LncRNA TUG1水平为0.76±0.15,低于未复发组1.01±0.24,差异有统计学意义,t=6.506,P<0.05;miR-142-5p水平为1.39±0.30,高于未复发组1.02±0.23,差异有统计学意义,t=8.812,P<0.05。复发组乳头凹陷患者比例为86.05%,高于未复发组68.45%,差异有统计学意义,χ^(2)=5.261,P<0.05;急性期患者比例为65.11%,高于未复发组(7.15%),差异有统计学意义,χ^(2)=5.261,P<0.05。血清LncRNA TUG1、miR-142-5p水平单独及联合预测浆细胞性乳腺炎复发的曲线下面积(AUC)分别为0.804、0.814、0.875。miR-142-5p(OR=2.713,95%CI:1.432~5.140,P<0.05)、分期(OR=2.825,95%CI:1.557~5.126,P<0.05)是浆细胞性乳腺炎患者复发的独立危险因素,LncRNA TUG1是浆细胞性乳腺炎患Objective To explore the predictive value of levels of serum long non-coding RNA taurine up-regulated gene 1(LncRNA TUG1)and microRNA-142-5p(miR-142-5p)for recurrence of plasma cell mastitis.Methods A total of 211 patients with plasma cell mastitis admitted to Shenzhen Longgang District Maternal and Child Health Care Hospital from 2019-01-01to 2022-10-01were included as the study subjects,and their clinical characteristics were collected.The patients were grouped into recurrent group(43cases)and non-recurrent group(168cases)according to whether they recurred after 1month of recovery.The levels of serumLncRNA TUG1and miR-142-5p were detected by real-time fluorescence quantitative PCR(qRT-PCR),the data were calculated using the 2-ΔΔCt method;The predictive value of serumLncRNA TUG1and miR-142-5p levels on the recurrence of plasma cell mastitis was analyzed by the ROC curve;Logistic regression analysis was applied to examine the influencing factors of recurrence of plasma cell mastitis.Results The serum LncRNA TUG1level in patients with plasma cell mastitis with depressed papillary state was 0.82±0.16,which was significantly lower than that in normal patients 1.32±0.21,t=18.594,P<0.05.The miR-142-5p level was 1.13±0.27,significantly higher than that of normal patients 1.01±0.20,t=3.098,P<0.05.The serumLncRNA TUG1level in patients with plasma cell mastitis in acute stage was 0.66±0.15,significantly lower than that in patients with subacute stage 0.96±0.21and chronic stage 1.11±0.22,and the difference was statistically significant,F=64.774,P<0.05.The miR-142-5p level was 1.37±0.28,significantly higher than that of patients in the subacute phase 1.11±0.27 and chronic phase 0.94±0.20,and the difference was statistically significant,F=40.428,P<0.05.The serum LncRNA TUG1level in the recurrent group was 0.76±0.15,significantly lower than that in the non recurrent group 1.01±0.24,and the difference was statistically significant,t=6.506,P<0.05.The miR-142-5p level was 1.39±0.30,significantly higher than the no

关 键 词：浆细胞性乳腺炎 长链非编码RNA牛磺酸上调基因1 微小RNA-142-5p 复发 

分 类 号：R655.8[医药卫生—外科学]