HPLC法同时测定连钱草中3个黄酮类成分和4个酚酸类成分的含量  

Simultaneous determination of three flavonoids and four phenolic acids in Glechomae Herba by HPLC

作　　者：廖圆月 袁铭铭 周雷罡 吴西 喻何云 LIAO Yuan-yue;YUAN Ming-ming;ZHOU Lei-gang;WU Xi;YU He-yun(Pharmacy Department,The People's Hospital of Ganzhou,Ganzhou 341000,China;Jiangxi Institute for Drug Control,NMPA Key Laboratory of Quality Evaluation of Traditional Chinese Patent Medicine,Jiangxi Province Engineering Research Center of Drug and Medical Device Quality Nanchang 330029,China)

机构地区：[1]赣州市人民医院药剂科,赣州341000 [2]江西省药品检验检测研究院国家药品监督管理局中成药质量评价重点实验室江西省药品与医疗器械质量工程技术研究中心,南昌330029

出　　处：《药物分析杂志》2023年第10期1664-1671,共8页Chinese Journal of Pharmaceutical Analysis

基　　金：江西省药品监督管理局科研项目(2021KY19);2022年度国家药品标准制修订研究课题(2022Z003);江西省重点研发计划项目(20171BBG70104)。

摘　　要：目的:建立同时测定连钱草中3个黄酮类成分(木犀草素-7-O-β-D-葡萄糖醛酸(1→2)-O-β-D-葡萄糖醛酸苷、芹菜素-7-O-β-D-葡萄糖醛酸(1→2)-O-β-D-葡萄糖醛酸苷、木犀草素)及4个酚酸类成分(绿原酸、隐绿原酸、咖啡酸、迷迭香酸)含量的高效液相色谱法。方法:采用Waters SunFire C18(250 mm×4.6 mm,5μm)色谱柱,以乙腈(A)-0.1%磷酸水溶液(B)为流动相,梯度洗脱,流速1.0 mL·min^(-1),检测波长330 nm,柱温30℃。结果:7个成分在各自范围内线性关系良好(r≥0.9996);精密度(RSD≤0.87%)、稳定性(RSD≤1.1%)良好;平均加样回收率(n=6)为99.1%~101.1%,RSD为0.59%~1.7%。18批样品中绿原酸、隐绿原酸、咖啡酸、木犀草素-7-O-β-D-葡萄糖醛酸(1→2)-O-β-D-葡萄糖醛酸苷、芹菜素-7-O-β-D-葡萄糖醛酸(1→2)-O-β-D-葡萄糖醛酸苷、迷迭香酸和木犀草素含量范围分别为0.046~0.602、0.071~0.817、0.102~1.159、0.728~4.817、0.592~4.081、0.850~13.060和0.019~0.198 mg·g^(-1)。结论:该方法简便易行,重复性好,可用于连钱草药材的质量控制。Objective:To develop an HPLC method for simultaneous determination of three flavonoids(luteolin7-O-β-glucuronyl-(1→2)-O-β-glucuronide,apigenin 7-O-β-glucuronyl-(1→2)-O-β-glucuronide and luteolin)and four phenolic acids(chlorogenic acid,cryptochlorogenic acid,caffeic acid and rosmarinic acid)in Glechomae Herba.Methods:The Waters SunFire C_(18)column(250 mm×4.6 mm,5μm)was used,the mobile phase was acetonitrile(A)-0.1% phosphoric acid(B)with gradient elution,at the flow rate of 1.0 mL·min^(-1),the detection wavelength was 330 nm,and the column temperature was 30℃.Results:Seven constituents showed good linear relationships within their own ranges(r≥0.9996),and the precision(RSD≤0.87%)and stability(RSD≤1.1%)were good.The average recoveries(n=6)were 99.1%-101.1% with the RSDs of 0.59%-1.7%.The contents of chlorogenic acid,cryptochlorogenic acid,caffeic acid,luteolin 7-O-β-glucuronyl-(1→2)-O-β-glucuronide,apigenin 7-O-β-glucuronyl-(1→2)-O-β-glucuronide,rosmarinic acid and luteolin in eighteen batches of samples were 0.046-0.602 mg·g^(-1),0.071-0.817 mg·g^(-1),0.102-1.159 mg·g^(-1),0.728-4.817 mg·g^(-1),0.592-4.081 mg·g^(-1),0.850-13.060 mg·g^(-1)and 0.019-0.198 mg·g^(-1),respectively.Conclusion:This method is specific,accurate and reproducible,and can be used to control the quality of the Glechomae Herba.

关 键 词：连钱草 酚酸类成分 黄酮类成分 迷迭香酸 木犀草素 含量测定 高效液相色谱法 

分 类 号：R917[医药卫生—药物分析学]