寒地野生茶条槭组培技术研究  

Tissue Culture Technology of Wild Acer ginala in Cold Regions

作　　者：舒钰 崔岩 SHU Yu;CUI Yan(Heilongjiang Forestry Science Research Institute,Harbin 150081,China;Changping District Bureau of Landscaping of Beijing Municipality,Beijing 102299,China)

机构地区：[1]黑龙江省林业科学研究所,黑龙江哈尔滨150081 [2]北京市昌平区园林绿化局,北京102299

出　　处：《黑龙江农业科学》2023年第11期89-94,共6页Heilongjiang Agricultural Sciences

基　　金：中央财政推广项目(黑〔2022〕TG10号)。

摘　　要：为了促进寒地野生茶条槭高效、稳定快速繁育,将东北地区丰富的野生槭树属资源用于选育优良彩叶树种,进而缓解我国北方寒地有观赏价值的特色种质极度匮乏的问题。以寒地野生茶条槭为材料,通过试验研究不同外植体采集时间对茶条槭茎段诱导的影响,消毒处理对外植体污染及褐化的影响,基本培养基筛选、细胞分裂素浓度与种类配比试验,细胞分裂素与生长素配比试验,探索寒地野生茶条槭的组培技术以及诱导培养基组培最佳配方。结果表明,寒地野生茶条槭组培外植体最佳采集时间为3月;最佳采集部位为下部茎段;最佳消毒时间为3 min;最佳培养基为MS;最佳增殖培养基配方为MS+6-BA 0.50 mg·L^(-1)+IBA 0.20 mg·L^(-1)+NAA 0.05 mg·L^(-1)+2%蔗糖+6 g·L^(-1)琼脂粉,pH6.2,愈伤组织诱导率可达89%。In order to promote efficient and stable rapid propagation of wild tea-leafed maple in cold regions,the abundant resources of wild maple trees in northeast China were utilized to breed excellent ornamental tree species,thereby alleviating the problem of extremely limited genetic resources of characteristic species in the cold regions of northern China.In this study,the cold-resistant wild tea-leafed maple was used as the material,the effects of different explant collection times on the induction of stem segments of tea-leafed maple,the effects of disinfection treatment on explant contamination and browning,basic medium screening,experiments on different ratios and concentrations of cytokinins and auxins,and experiments on the ratio of cytokinins to auxins were studied to explore the tissue culture technique of cold-resist ant wild tea-leafed maple and the optimal formula for inducing culture medium for tissue culture.The results showed that the optimal collection time for tissue culture explants of cold-resistant wild tea-leafed maple was in March,the optimal collection part was the lower stem segment,the optimal disinfection time was 3 minutes.The optimal medium was MS and the optimal formula for proliferating medium was MS+6-BA 0.50 mg·L^(-1)+IBA 0.20 mg·L^(-1)+NAA 0.05 mg·L^(-1)+2%sucrose+6 g·L^(-1) agar,pH was 6.2,and the induction rate of callus tissue could reach 89%.

关 键 词：茶条槭 寒地 野生 组织培养 

分 类 号：S687[农业科学—观赏园艺]