浮游态与生物被膜态鸡源大肠杆菌耐药性及功能性差异的研究  

Investigation of drug resistance and functional difference between planktonic and biofilm forms of Escherichia coli in chicken

作　　者：陶梦珂 赵恒 石晴晴 张鲁星 李苗苗 胡功政[1] 苑丽[1] 刘建华[1] TAO Meng-ke;ZHAO Heng;SHI Qing-qing;ZHANG Lu-xing;LI Miao-miao;HU Gong-zheng;YUAN Li;LIU Jian-hua(College of Veterinary Medicine,Henan Agricultural University,Zhengzhou 450046,China)

机构地区：[1]河南农业大学动物医学院,河南郑州450046

出　　处：《中国兽医科学》2023年第11期1461-1467,共7页Chinese Veterinary Science

基　　金：国家自然科学基金面上项目(32072914);河南省重点研发项目(221111111300)。

摘　　要：为了解大肠杆菌在生物被膜形成前后的耐药性及功能差异,进一步探究生物被膜导致的耐药机制,本研究测定并比较了阿米卡星、头孢噻呋、多西环素、氟苯尼考、环丙沙星、庆大霉素、黏菌素7种抗菌药物对EL8、EM3及ES10株大肠杆菌在生物被膜态和浮游态的最小抑菌浓度(MIC)、最小杀菌浓度(MBC),并对两种状态下的3株细菌进行疏水性、菌体自聚性、内外膜通透性、ATP含量、活性氧水平及外排泵功能进行了测定。结果显示,细菌生物被膜态的最小抑菌浓度、最小杀菌浓度高于浮游态,分别增大至1.25~160倍、1.25~640倍。与浮游态相比,EL8、EM3及ES10株细菌在生物被膜态的疏水性和菌体自聚合能力均有所增强,且差异显著(P<0.05)。EM3、ES10株细菌生物被膜态的内外膜通透性和活性氧水平均强于浮游态,差异显著(P<0.05)。一系列功能性研究发现生物被膜形成后,EL8、EM3及ES10株细菌ATP含量升高、氧化损伤能力及外排泵功能增强。以上结果表明无论成膜能力强弱,在生物被膜形成后,其耐药性及细菌功能均有所增强。In order to understand the drug resistance and functional differences before and after the formation of Escherichia coli(E.coli)biofilm,and further explore the mechanism of drug resistance caused by biofilm,the minimum inhibitory concentration(MIC)and minimum bactericidal concentration(MBC)of amikacin,ceftiofur,doxycycline,flufenicol,ciprofloxacin and colistin against EL8,EM3 and ES10 strains of E.coli were determined and compared.Additionally,the hydrophobicity,autoaggregation ability,inner and outer membrane permeability,ATP content,reactive oxygen species level and efflux pump function were assessed.The results showed that the minimum inhibitory and bactericidal concentrations in biofilm state were higher than those in planktonic state,increasing to 1.25—160 times and 1.25—640 times,respectively.Compared with the planktonic state,the hydrophobicity and autoaggregation ability of EL8,EM3 and ES10 strains of E.coli in the biofilm state were enhanced,and the differences were significant(P<0.05).Both inner and outer membrane permeability and reactive oxygen species(ROS)levels of EM3 and ES10 strains of E.coli in biofilm state were significantly higher than those in planktonic state(P<0.05).A series of functional studies showed that ATP content,oxidative damage ability and efflux pump function of EL8,EM3 and ES10 strains of E.coli increased after biofilm formation.The above results indicated that drug resistance and bacterial function were enhanced after biofilm formation,regardless of their biofilm formation capacity.

关 键 词：大肠杆菌 生物被膜态 浮游态 MIC 内外膜通透性 细菌功能 

分 类 号：S852.612[农业科学—基础兽医学]