基于泡腾辅助脂肪酸形态转化的悬浮固化-分散液液微萃取测定水样中环境雌激素  

An optimized of effervescence-assisted switchable fatty acid-based microextraction with solidification of floating organic droplet for determination of environmental estrogens in environmental water samples

作　　者：陈瑾[1] 刘媚媚 曲晋刚 CHEN Jin;LIU Meimei;QU Jingang(Zhejiang Tongji Vocational College of Science and Technology,Hangzhou,311231,China;Zhejiang Hongbo Environmental Testing Co.,Ltd,Hangzhou,311100,China;Huiyou(Hangzhou)Pharmaceutical Technology Service Co.,Ltd,Hangzhou,310020,China)

机构地区：[1]浙江同济科技职业学院,杭州311231 [2]浙江鸿博环境检测有限公司,杭州311100 [3]辉侑(杭州)医药技术服务有限公司,杭州310020

出　　处：《环境化学》2023年第11期4043-4046,共4页Environmental Chemistry

摘　　要：本研究建立脂肪酸形态转化液液微萃取技术并结合高效液相色谱对湖水和河水中雌酮、己烯雌酚和己烷雌酚这3种环境雌激素(EEs)进行了测定.利用脂肪酸可在亲水-疏水形态间转换的特点,对5种中链脂肪酸筛选得到辛酸作为萃取剂,同时根据其低温固化的特点,利用漂浮液滴固化微萃取技术进行测定.通过实验设计优化最优萃取条件为:H_(2)SO_(4)(98%)体积238μL、Na_(2)CO_(3)(2 mol·L^(-1))体积413μL、脂肪酸体积144μL和涡旋时间3.3 min.最优萃取条件下检测,定量限范围0.110-0.201μg·L^(-1),回收率范围89.0%—101.5%.本方法具有简便快捷,检测限低,回收率高,绿色环保等优点,适用于多种环境水体中EEs的快速定性及定量分析.This study created a new method for easily and quickly determining environmental estrogens(estrone,diethylstilbestrol,and hexestrol)in samples of tap water,lake water,rainwater,and river water.This method uses a switchable fatty acid-based microextraction assisted by effervescence in conjunction with the solidification of a floating organic droplet.Five medium-chain fatty acids were tested as an extraction solvent given their ability to change between hydrophobic and hydrophilic forms by pH adjustment.Octanoic acid had the capacity to change from a liquid to a solidified floating state at low temperatures and had outstanding extraction recovery for three environmental estrogens,therefore four primary characteristics were assessed using central composite design as follows:238μL H_(2)SO_(4)(98%),413μL Na_(2)CO_(3)(2 mol·L^(-1)),144μL fatty acid and 3.3 min vortex time.Under optimized conditions,limits of quantitation were 0.110—0.201µg·L^(-1) and extraction recoveries were 89.0%—101.5%for three environmental estrogens in four kinds of environmental water samples.Overall,the new method is simple,quick,and environment-friendly with low detection limits and high recoveries.

关 键 词：环境雌激素 中链脂肪酸 形态转换微萃取 环境水体样品 响应面优化 

分 类 号：X832[环境科学与工程—环境工程]