生物3D打印研究进展:动物、植物及微生物细胞的增材制造  

Advances in 3D Bioprinting:Additive Manufacturing of Living Cells from Animal,Plant,and Microbe

作　　者：李佩锡 周德志 杨长明 饶玮祎 林峰[1] 欧阳礼亮 LI Peixi;ZHOU Dezhi;YANG Changming;RAO Weiyi;LIN Feng;OUYANG Liliang(Department of Mechanical Engineering,Tsinghua University,Beijing 100084;Weiyang College,Tsinghua University,Beijing 100084;Tanwei College,Tsinghua University,Beijing 100084)

机构地区：[1]清华大学机械工程系,北京100084 [2]清华大学未央书院,北京100084 [3]清华大学探微书院,北京100084

出　　处：《机械工程学报》2023年第19期237-252,共16页Journal of Mechanical Engineering

摘　　要：生物有机体的仿生制造对人类探索和运用生命机制具有重要意义。生物3D打印基于计算机三维模型,精准控制活细胞、生物材料、生化因子等物质的空间位置,可以模拟人体的组织结构,制造具有复杂异质性的仿生生物组织,并应用于基础研究和临床转化。近年来,生物3D打印技术还拓展到了植物细胞培养以及微生物合成发酵等领域,展现出巨大的应用潜力。本文将对生物3D打印技术的基本原理及最新进展进行介绍,并从动物、植物和微生物细胞打印三方面综述应用现状及挑战,以期对广义生物3D打印的技术发展及应用趋势进行全面梳理。The bionic manufacturing of biological organisms is of great significance to the exploration and exploitation of life mechanisms.Based on computer-aided design and control,3D bioprinting can spatially manipulate living cells,biomaterials,biochemical factors,and other substances.It can produce biological tissue constructs that mimic the structural complexity and component heterogeneity of native tissues for fundamental research in life science and clinical translation in medicine.In recent years,3D bioprinting technology has also been used in plant cell culture and microbial synthesis with great potential.This paper will introduce the basic principles and recent advances of 3D bioprinting technologies,and overview the applications of bioprinting animal,plant,and microbial cells by providing the most recent examples.This paper will also discuss the challenges and opportunities and aims to provide a vision for the trends in the field.

关 键 词：生物3D打印 动物细胞打印 植物细胞打印 微生物细胞打印 

分 类 号：TG156[金属学及工艺—热处理]