灵芝子实体中18种主要三萜成分的高效液相分析  

Analysis of 18 main triterpenoids in basidiomata of Ganoderma lingzhi by HPLC

作　　者：曾凡清 吕明亮 薛振文 冯娜[2] ZENG Fanqing;LÜMingliang;XUE Zhenwen;FENG Na(Lishui Institute of Agricultural and Forestry Sciences,Lishui 323000,Zhejiang,China;National Engineering Research Center of Edible Fungi,Key Laboratory of Edible Fungal Resources and Utilization(South),Ministry of Agriculture and Rural Affairs of the People’s Republic of China,Key Laboratory of Agricultural Genetics and Breeding of Shanghai,Institute of Edible Fungi,Shanghai Academy of Agricultural Sciences,Shanghai 201403,China Abstract:Using 18 main triterpenoids as references,a high-performance liquid chromatography)

机构地区：[1]丽水市农林科学研究院,浙江丽水323000 [2]上海市农业科学院食用菌研究所国家食用菌工程技术研究中心农业农村部南方食用菌资源利用重点实验室上海市农业遗传育种重点开放实验室,上海201403

出　　处：《菌物学报》2023年第11期2309-2319,共11页Mycosystema

基　　金：丽水市重点研发计划(2012ZDYF11)。

摘　　要：以灵芝子实体中18种主要三萜化合物为对照品,建立了灵芝三萜的高效液相定量检测方法。采用Agilent Eclipse Plus C18色谱柱(4.6 mm×250 mm,5μm),以乙腈-水(0.01%乙酸)为流动相进行梯度洗脱,柱温30℃,流速1.0 mL/min,检测波长254 nm。该方法实现了18种三萜化合物的基线分离,精密度、重复性、稳定性和加样回收率均符合方法学要求。将该方法应用于2个灵芝品种在不同栽培环境下的三萜含量评价中,结果发现,三萜的含量与品种、栽培环境都有很大的相关性,品种是影响灵芝子实体三萜含量的主要因素,但在不同环境下,同一品种的灵芝三萜含量也会发生变化。Using 18 main triterpenoids as references,a high-performance liquid chromatography(HPLC)method for quantitative determination of triterpenoids in basidiomata of Ganoderma lingzhi was established.HPLC was carried out by Agilent Eclipse Plus C18 column(4.6 mm×250 mm,5μm)with acetonitrile-0.01%acetic acid system as mobile phase.The column temperature was 30°C,the flow rate was 1.0 mL/min,and the detective wavelength was set at 254 nm.The HPLC method was applied to the evaluation of triterpenoid content in the two strains of G.lingzhi under different cultivation environments.It was found that triterpenoid content was significantly correlated with both the strains and the cultivation environment.Strains were the main factor affecting the triterpenoid content in basidiomata of G.lingzhi,but the triterpenoid content also changed under different environments.

关 键 词：灵芝子实体 三萜 定量分析 种质评价 

分 类 号：R284[医药卫生—中药学]