The Dof transcription factor COG1 acts as a key regulator of plant biomass by promoting photosynthesis and starch accumulation  

作　　者：Zhuoyun Wei Haoyong Zhang Meng Fang Shuyuan Lin Mingsong Zhu Yuxiu Li Limin Jiang Tianliang Cui Yanwei Cui Hong Kui Liang Peng Xiaoping Gou Jia Li 

机构地区：[1]Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations,School of Life Sciences,Lanzhou University,Lanzhou 730000,China [2]Gansu Key Laboratory of Gene Editing for Breeding,School of Life Sciences,Lanzhou University,Lanzhou 730000,China [3]Gansu Key Laboratory of Biomonitoring and Bioremediation for Environmental Pollution,School of Life Sciences,Lanzhou University,Lanzhou 730000,China [4]Guangdong Provincial Key Laboratory of Plant Adaptation and Molecular Design,School of Life Sciences,Guangzhou University,Guangzhou 510006,China

出　　处：《Molecular Plant》2023年第11期1759-1772,共14页分子植物（英文版）

基　　金：supported in part by the National Natural Science Foundation of China(32270341,31700245,and 32030005);the Natural Science Foundation for Distinguished Young Scholars of Gansu Province(20JR5RA306);the Postdoctoral Research Foundation of China(2018T111116 and 2016M602889);the Programme of Introducing Talents of Discipline to Universities(B16022);the Fundamental Research Funds for the Central Universities(lzujbky-2020-32 and lzujbky-2022-kb03).

摘　　要：Photosynthetic efficiency is the primary determinant of crop yield,including vegetative biomass and grain yield.Manipulation of key transcription factors known to directly control photosynthetic machinery can be an effective strategy to improve photosynthetic traits.In this study,we identified an Arabidopsis gain-of-function mutant,cogwheel1-3D,that shows a significantly enlarged rosette and increased biomass compared with wild-type plants.Overexpression of COG1,a Dof transcription factor,recapitulated the phenotype of cogwheel1-3D,whereas knocking out COG1 and its six paralogs resulted in a reduced rosette size and decreased biomass.Transcriptomic and quantitative reverse transcription polymerase chain reaction analyses demonstrated that COG1 and its paralogs were required for light-induced expression of genes involved in photosynthesis.Further chromatin immunoprecipitation and electrophoretic mobility shift assays indicated that COG1 can directly bind to the promoter regions of multiple genes encoding light-harvesting antenna proteins.Physiological,biochemical,and microscopy analyses revealed that COG1 enhances photosynthetic capacity and starch accumulation in Arabidopsis rosette leaves.Furthermore,combined results of bioinformatic,genetic,and molecular experiments suggested that the functions of COG1 in increasing biomass are conserved in different plant species.These results collectively demonstrated that COG1 acts as a key regulator of plant biomass by promoting photosynthesis and starch accumulation.Manipulating COG1 to optimize photosynthetic capacity would create new strategies for future crop yield improvement.

关 键 词：COG1 Dof transcription factor LHCA LHCB photosynthetic genes BIOMASS 

分 类 号：Q94[生物学—植物学]