淫羊藿苷对白细胞介素-1β刺激的软骨细胞增殖、自噬和炎症反应的影响  

Effects of icariin on interleukin-1βstimulated chondrocyte proliferation,autophagy and inflammatory response

作　　者：颜荣耀 曾志远[1] 王浩 蔡振煜 YAN Rong-yao;ZENG Zhi-yuan;WANG Hao;CAI Zhen-yu(Department of Orthopedics,Quanzhou First Hospital Affiliated to Fujian Medical University,Quanzhou 362000,Fujian Province,China)

机构地区：[1]福建医科大学附属泉州第一医院骨科,福建泉州362000

出　　处：《中国临床药理学杂志》2023年第22期3301-3305,共5页The Chinese Journal of Clinical Pharmacology

摘　　要：目的研究淫羊藿苷(ICA)对白细胞介素(IL)-1β刺激的软骨细胞增殖、自噬和炎症反应的影响。方法将人软骨细胞C28/I2分为对照组(空白培养)、IL-1β组(10 ng·mL^(-1)IL-1β处理)和淫羊藿苷低、中、高剂量组(10 ng·mL^(-1)IL-1β处理细胞后分别用50、100、150μg·mL^(-1)淫羊藿苷处理),以细胞计数试剂盒(CCK-8)法检测细胞存活率,以蛋白质印迹法(Western blot)检测细胞周期蛋白D1(Cyclin D1)、自噬相关蛋白(Beclin)-1、自噬相关基因7(ATG7)及核因子-κB(NF-κB)通路相关蛋白表达,以酶联免疫吸附法检测肿瘤坏死因子-α(TNF-α)、IL-6、IL-8水平。结果对照组、IL-1β组和淫羊藿苷低、中、高剂量组的24 h细胞存活率分别为(100.32±6.89)%、(44.31±5.74)%、(63.97±7.32)%、(75.39±9.85)%、(89.24±6.58)%,48 h细胞存活率分别为(100.02±5.32)%、(36.89±4.24)%、(59.43±3.85)%、(67.28±8.71)%、(79.43±8.44)%,TNF-α水平分别为(108.59±13.11)、(669.43±82.57)、(589.74±56.87)、(421.30±49.87)、(242.15±35.41)pg·mL^(-1),IL-6水平分别为(62.71±6.85)、(324.15±39.47)、(285.45±30.18)、(194.74±21.26)、(123.54±13.12)pg·mL^(-1),IL-8水平分别为(45.78±6.78)、(214.53±26.27)、(157.32±14.85)、(98.74±11.32)、(60.18±7.81)pg·mL^(-1)。上述指标,对照组与IL-1β组比较,差异均有统计学意义(均P<0.05);IL-1β组与淫羊藿苷低、中、高剂量组比较,差异均有统计学意义(均P<0.05)。结论淫羊藿苷可减轻IL-1β刺激的软骨细胞自噬和炎症反应,并诱导细胞增殖。Objective To investigate the effects of icariin(ICA)on chondrocyte proliferation,autophagy and inflammatory response stimulated by interleukin-1β(IL-1β).Methods Human chondrocytes C28/I2 were divided into control group(blank culture),IL-1βgroup(IL-1βconcentration 10 ng·mL^(-1))and ICA-L,ICA-M,ICA-H groups(cells treated with IL-1βconcentration of 10 ng·mL^(-1)were treated with 50,100,150μg·mL^(-1)icariin).Cell survival rate was detected by cell counting kit-8(CCK-8);the expressions of CyclinD1,Beclin-1,autophagy associated gene 7(ATG7)and proteins related to nuclear factor-κB(NF-κB)pathway were detected by Western blot;the levels of tumor necrosis factor-α(TNF-α),IL-6 and IL-8 were detected by enzyme-linked immunosorbent assay.Results The 24 h cell survival rates of control group,IL-1βgroup,ICA-L group,ICA-M group and ICA-H group were(100.32±6.89)%,(44.31±5.74)%,(63.97±7.32)%,(75.39±9.85)%,(89.24±6.58)%;cell survival rate at 48 h were(100.02±5.32)%,(36.89±4.24)%,(59.43±3.85)%,(67.28±8.71)%,(79.43±8.44)%;TNF-αlevels were(108.59±13.11),(669.43±82.57),(589.74±56.87),(421.30±49.87),(242.15±35.41)pg·mL^(-1);IL-6 levels were(62.71±6.85),(324.15±39.47),(285.45±30.18),(194.74±21.26),(123.54±13.12)pg·mL^(-1);IL-8 levels were(45.78±6.78),(214.53±26.27),(157.32±14.85),(98.74±11.32),(60.18±7.81)pg·mL^(-1).The above indexes were statistically significant between control group and IL-1βgroup(all P<0.05);IL-1βgroup was significantly different from ICA-L group,ICA-M group and ICA-H group(all P<0.05).Conclusion Icariin can reduce IL-1β-stimulated chondrocyte autophagy and inflammatory response,and induce cell proliferation.

关 键 词：淫羊藿苷 白细胞介素-1Β 软骨细胞 增殖 自噬 炎症反应 

分 类 号：R28[医药卫生—中药学]