新疆野生樱桃李PsoRPm2与PsoLFY互作调控植株开花的分子机制  

Molecular mechanism of interaction between PsoRPM2and PsoLFY in regulating the flowering of Xinjiang wild myrobalan plum(Prunus sogdiana)

作　　者：高朝远 关平印 蒲文江 袁梓珍 罗嗣芳 胡建芳 GAO Chaoyuan;GUAN Pingyin;PU Wenjiang;YUAN Zizhen;LUO Sifang;HU Jianfang(College of Horticulture,China Agricultural University,Beijing 100193,China)

机构地区：[1]中国农业大学园艺学院,北京100193

出　　处：《中国农业大学学报》2023年第11期103-115,共13页Journal of China Agricultural University

基　　金：国家自然科学基金项目(31972360)。

摘　　要：为明确新疆野生樱桃李PsoRPm2基因引起植株的早开花现象,本研究通过形态学、转基因以及酵母双杂等试验对PsoRPm2基因进行了分析。结果显示:新疆野生樱桃李极早花、早花、中花、晚花和极晚花的比率分别为11%、24%、44%、18%和3%。早花类型的花芽分化明显快于晚花类型,早花花芽中的PsoLFY和PsoFT基因表达高于晚花,而PsoFLC基因则在晚花中表达较高。转PsoRPm2基因烟草表现为早花,并且NtLFY基因表达明显升高。在新疆野生樱桃李早花花芽中,PsoRPm2基因表达高于晚花,同一时期的PsoLFY基因表达也较高。酵母双杂试验显示,PsoRPm2与PsoLFY可以互作。综上,新疆野生樱桃李之所以早花是通过PsoRPm2与PsoLFY互作,提高花芽中PsoLFY和PsoFT基因表达,从而加快花芽分化进程,导致植株提前开花。In order to clarify the regulatory effect of Xinjiang wild myrobalan plum cloned gene PsoRPM2 on the early flowering of plants,the function of early flowering caused by PsoRPM2 gene was analyzed by morphological,transgenic and yeast two-hybrid experiments.The result revealed that:The percentage of myrobalan plum seedlings with extremely early,early,middle,late and very late flowering characters were 11%,24%,44%,18%and 3%,respectively.The differentiation of floral bud in the early flowering type was significantly faster than that in the late flowering type.The expressions of PsoLFYand PsoFT genes in flower buds of early-flowering plants were significantly higher than that in late-flowering plants,while PsoFLC was higher in late-flowering plants.Early flowering was also found in the transgenic tobacco lines with PsoRPM2,and NtLFY gene expression was significantly increased.In Xinjiang wild myrobalan plum,the expression of psoRPM2 gene was higher than that in late-flowering plants,and the expression of PsoLFY was also higher in floral buds of early-flowering plants at the same period.Yeast two-hybrid assay showed that PsoRPM2and PsoLFY interacted with each other.In summary the interaction between PsoRPM2and PsoLFY may increase the expression of PsoLFY and PsoFT in flower buds,thereby accelerating floral bud differentiation process and leading the early flowering of plants.

关 键 词：新疆野生樱桃李 早花 转PsoRPm2基因 PsoLFY基因 酵母双杂 

分 类 号：S662.3[农业科学—果树学]