孕康口服液对脾虚妊娠小鼠的作用研究  

Effect of Yunkang Oral Solution on pregnant mice with spleen deficiency syndrome

作　　者：汪晨兴 颜美秋 苏洁[1] 俞静静 郭静妍 李婷 田雅娟 牛壮伟 陈素红[2] 吕圭源[1] WANG Chen-xing;YAN Mei-qiu;SU Jie;YU Jing-jing;GUO Jing-yan;LI Ting;TIAN Ya-juan;NIU Zhuang-wei;CHEN Su-hong;LYU Gui-yuan(School of Pharmaceutical Sciences,Zhejiang Chinese Medical University,Hangzhou 310053,China;Zhejiang University of Technology,Hangzhou 310014,China)

机构地区：[1]浙江中医药大学药学院,浙江杭州310053 [2]浙江工业大学,浙江杭州310014

出　　处：《中国中药杂志》2023年第20期5592-5602,共11页China Journal of Chinese Materia Medica

基　　金：浙江省省级重点实验室项目(2012E10002)。

摘　　要：研究孕康口服液对饮食不节结合过食苦寒致脾虚妊娠小鼠脾虚状态以及妊娠结局的改善作用。模拟人类饮食不节、过食苦寒致脾虚,实验采用隔日断食隔日给予高脂饲料复合番泻叶灌胃法制备脾虚妊娠小鼠。实验期间测定脾虚相关表征及腹泻相关指标;测定胃肠动力(胃排空率及小肠推进率);检测血清胃饥饿素(ghrelin)、生长激素(growth hormone,GH)、胃泌素(gastrin,Gas)、总胆固醇(total cholesterol,TC)、低密度脂蛋白胆固醇(low-density lipoprotein cholesterol,LDL-c)、绒毛膜促性腺激素β(chorionic gonadotropin β,β-CG)、孕酮(progesterone,P)及雌二醇(estradiol,E_(2))的含量;评价脾虚妊娠小鼠肠屏障功能;计算脾虚妊娠小鼠受孕率、卵巢系数、连胎子宫系数、活胎数、胎鼠平均体质量及胎鼠身长。结果显示,孕康口服液可显著改善脾虚妊娠小鼠脾虚相关表征及腹泻情况;增加胃排空率和小肠推进率;升高血清中胃肠激素(ghrelin、GH、Gas)水平;降低血清中脂质水平(TC、LDL-c),从而改善脂质代谢紊乱;改善结肠组织形态,增加杯状细胞数量,同时促进结肠组织中occludin、claudin-1 mRNA及蛋白的表达从而减轻肠屏障受损。孕康口服液还能调节脾虚妊娠小鼠血清中妊娠激素(β-CG、P、E_(2))的含量,同时提高受孕率、卵巢系数、连胎子宫系数、活胎数、胎鼠平均体质量及胎鼠身长;提示孕康口服液能改善脾虚妊娠小鼠妊娠前及妊娠期间脾虚相关症状,调节妊娠相关激素从而改善妊娠结局。This study aimed to investigate the therapeutic effect of Yunkang Oral Solution on the improvement of spleen deficiency and pregnancy outcomes in pregnant mice with spleen deficiency syndrome induced by irregular diet and over consumption of cold and bitter foods.To simulate human irregular diet and over consumption of cold and bitter foods leading to spleen deficiency,the pregnant mice with spleen deficiency syndrome were prepared using an alternate-day fasting and high-fat diet combined with oral administration of Sennae Folium.During the experiment,spleen deficiency-related indicators and diarrhea-related parameters were measured.Gastric and intestinal motility(gastric emptying rate and intestinal propulsion rate) were evaluated.The levels of serum ghrelin,growth hormone(GH),gastrin(Gas),total cholesterol(TC),low-density lipoprotein cholesterol(LDL-c),chorionic gonadotropin β(β-CG),progesterone(P),and estradiol(E_2) were measured.Intestinal barrier function in pregnant mice with spleen deficiency syndrome was assessed.Conception rate,ovarian coefficient,litter-bearing uterine coefficient,number of live fetuses,average fetal weight,and fetal length were calculated.The results showed that Yunkang Oral Solution significantly improved spleen deficiency-related indicators and diarrhea in pregnant mice with spleen deficiency syndrome,increased gastric emptying rate and intestinal propulsion rate,elevated the levels of gastrointestinal hormones(ghrelin,GH,and Gas) in the serum,and reduced lipid levels(TC and LDL-c),thereby improving lipid metabolism disorders.It also improved colonic tissue morphology,increased the number of goblet cells,and promoted the mRNA and protein expression of occludin and claudin-1 in colonic tissues,thereby alleviating intestinal barrier damage.Yunkang Oral Solution also regulated the levels of pregnancy hormones(β-CG,P,and E_2) in the serum of pregnant mice with spleen deficiency syndrome.Moreover,it increased the conception rate,ovarian coefficient,litter-bearing uterine coefficient,nu

关 键 词：孕康口服液 脾虚 妊娠 妊娠结局 肠屏障 

分 类 号：R285.5[医药卫生—中药学]