基于多参数流式细胞术精细化分析外周血免疫细胞亚群的中国专家共识  

The consensus of Chinese experts on refined analysis of immune cell subsets in peripheral blood by multi-parameter flow cytometry

作　　者：中国中西医结合学会检验医学专业委员会 王卉 崔巍 刘贵建[4] 陈曼 李壹[6] 庞博[7] 汪峰 王慧君 王建中[10] 王雅杰[11] 吴雨洁 朱明清[14] Laboratory Medicine Committee of Chinese Association of Integrative Medicine;Wang Hui;Cui Wei;Liu Gujian(不详;Department of Clinical Laboratory,Hebei Yanda Lu Daopei Hospital,Langfang 065201,China;Department of Clinical Laboratory,National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100021,China;Department of Clinical Laboratory,Guang'anmen Hospital,China Academy of Chinese Medical Sciences,Beijing 100053,China)

机构地区：[1]不详 [2]河北燕达陆道培医院检验科,廊坊065201 [3]国家癌症中心/国家肿瘤临床医学研究中心/中国医学科学院北京协和医学院肿瘤医院检验科,北京100021 [4]中国中医科学院广安门医院检验科,北京100053 [5]河北燕达陆道培医院检验科 [6]四川大学华西医院实验医学科 [7]中国中医科学院广安门医院检验科 [8]华中科技大学同济医学院附属同济医院检验科 [9]中国医学科学院血液病医院血液病理诊断中心 [10]北京大学第一医院 [11]首都医科大学附属地坛医院检验科 [12]南京医科大学第一附属医院 [13]江苏省人民医院血液科 [14]苏州大学附属第一医院江苏省血液研究所

出　　处：《中华预防医学杂志》2023年第11期1729-1747,共19页Chinese Journal of Preventive Medicine

摘　　要：免疫细胞亚群检测在多种良恶性疾病的临床诊疗及健康管理中都起着非常重要的作用。为了更好地开展不同功能性免疫细胞亚群的深入研究和建立克隆性相关指标参考区间,建立免疫衰老、个体化动态监测和治疗恢复期特殊参考区间,发现淋巴细胞以外其他免疫细胞的临床意义等,迫切需要精细化分析外周血免疫细胞亚群。多参数流式细胞术是检测免疫细胞精细化亚群、进行免疫功能评价的重要技术方法,为规范外周血免疫细胞亚群的流式细胞术精细化检测方法和方案,进一步促进其在临床疾病诊疗和健康管理中的应用,中国中西医结合学会检验医学专业委员会组织专家制定了此专家共识。The detection of immune cell subsets plays a very important role in the clinical diagnosis and treatment of various benign and malignant diseases and health management.In order to better carry out in-depth research on different functional immune cell subsets,establish reference intervals for clonality related indicators,establish special reference intervals for immune aging,individualized dynamic monitoring and treatment recovery,and discover the clinical significance of immune cells other than lymphocytes,it is urgent to analyze the peripheral blood immune cell subsets in a refined way.Multiparameter flow cytometry is an important technical method to detect immune cell subsets and evaluate immune function.In order to standardize the refined detection methods and protocols of peripheral blood immune cell subsets by flow cytometry,and further promote its application in clinical diagnosis and treatment of diseases and health management,Laboratory Medicine Committee of Chinese Association of Integrative Medicine(LMC-CAIM)organized experts to formulate this expert consensus.

关 键 词：免疫细胞 精细化分析 流式细胞术 多参数 共识 

分 类 号：R446.1[医药卫生—诊断学]