当归六黄汤质量标志物的发现及其作用机制解析研究  

Study on Discovery of Quality Markers for Danggui Liuhuang Decoction and Its Action Mechanism

作　　者：陈兰茹 马天宇 毛宏宇 黄家豪 孟永海[1] 翟春梅[1] Chen Lanru;Ma Tianyu;Mao Hongyu;Huang Jiahao;Meng Yonghai;Zhai Chunmei(Key Laboratory of Basic and Applied Research of Northen Medicine(Ministry of Education),Heilongjiang University of Chinese Medicine,Harbin 150006,China)

机构地区：[1]黑龙江中医药大学教育部北药基础与应用研究重点实验室,哈尔滨150006

出　　处：《中国药事》2023年第11期1296-1309,共14页Chinese Pharmaceutical Affairs

基　　金：哈尔滨市科技创新人才研究专项资金项目(编号2017RAQXJ125);黑龙江中医药大学研究生创新科研项目(编号2022yjscx055);黑龙江省博士后科研启动金资助项目(编号LBH-Q15137)。

摘　　要：目的:基于中药质量标志物(Q-marker)“五原则”和网络药理学,为当归六黄汤的质量控制研究提供参考,并为其临床应用提供科学依据。方法:根据Q-marker“五原则”对当归六黄汤的质量标志物进行筛选,再利用中药系统药理学数据库(TCMSP)、ChEMBL与Swiss Target Prediction平台获取功效标志物的潜在作用靶点,构建当归六黄汤质量标志物的蛋白互作网络。利用拓扑、GO、KEGG富集分析构建“关键靶点-信号通路-生物过程”网络,进而阐释当归六黄汤质量标志物的作用机制。结果:根据“五原则”和网络药理学综合分析,筛选阿魏酸、黄芪甲苷、梓醇、汉黄芩素、地黄苷D、黄芩苷、黄柏碱、汉黄芩苷、小檗碱作为当归六黄汤的质量标志物,这些标志物可能通过作用于MAPK、雌激素、PI3K-Akt等通路从而调节MAPK级联、基因表达、一氧化氮生物合成过程抑制炎症。结论:本研究初步探究当归六黄汤的质量标志物及其作用机制,为当归六黄汤的机制研究和临床应用提供更有利的科学依据与理论基础。Objective:Based on the"five principles"of traditional Chinese medicine quality markers(Q-marker)and network pharmacology,to provide reference for the quality control of Danggui Liuhuang Decoction,and to provide scientific basis for its clinical application.Methods:The quality markers of Danggui Liuhuang Decoction were screened according to the"five principles"of Q-markers,and the potential targets of the efficacy markers were obtained by using the TCM System Pharmacology Database(TCMSP),ChEMBL and Swiss Target Prediction platform.And the Protein-protein Interaction network of quality markers of Danggui Liuhuang Decoction was constructed.Topology,GO and KEGG enrichment analyses were used to construct the"key target-signal pathway-biological process"network,and then the mechanism of the quality markers of Danggui Liuhuang Decoction was explained.Results:According to the"five principles"and network pharmacological analysis,ferulic acid,astragaloside,catalpol,wogonin,rehmannioside D,baicalin,phellodendrine,wogonoside and berberine were selected as quality markers for Danggui Liuhuang Decoction.These markers may regulate inflammation by acting on MAPK,estrogen,PI3K-Akt and other pathways,thereby regulating the MAPK cascade,gene expression and nitric oxide biosynthesis.Conclusion:This study preliminarily explore the quality markers of Danggui Liuhuang Decoction and its mechanism of action.It provides more favorable scientific basis and theoretical basis for the mechanism research and clinical application of Danggui Liuhuang Decoction.

关 键 词：当归六黄汤 质量标志物 作用机制 网络药理学 

分 类 号：R932[医药卫生—生药学] R96[医药卫生—药学]