两种金小蜂类icarapin蛋白潜在致敏性的初步评价  

Evaluation of the Potential Allergenicity of Icarapin-like Proteins of Two Pteromalid Wasps

作　　者：曹昀珅 杨义 方琦[1] 汪芳[1] 叶恭银[1] CAO Yunshen;YANG Yi;FANG Qi;WANG Fang;YE Gongyin(State Key Laboratory of Rice Biology/Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insect Pests of Zhejiang Province/Institute of Insect Sciences,Zhejiang University,Hangzhou 310058,China)

机构地区：[1]浙江大学昆虫科学研究所/水稻生物育种全国重点实验室/农业部作物病虫害分子生物学重点实验室,杭州310058

出　　处：《中国生物防治学报》2023年第5期1049-1057,共9页Chinese Journal of Biological Control

基　　金：国家自然科学基金(31830074)。

摘　　要：西方蜜蜂icarapin蛋白是一种常见的人畜过敏原,在寄生蜂类群中也有其同源物存在。为探究寄生蜂中类icarapin蛋白的潜在致敏性,本文克隆并表达了蝶蛹金小蜂和丽蝇蛹集金小蜂中类icarapin蛋白编码基因。序列分析表明,两种金小蜂类icarapin蛋白编码基因开放阅读框分别为645 bp和714 bp,生物信息学预测其均具有潜在的致敏性。经Ni离子亲和层析柱纯化得到了高纯度的重组类icarapin蛋白,在此基础上建立了小鼠致敏模型,初步探究了其潜在致敏性。结果表明,注射2种寄生蜂和西方蜜蜂重组icarapin蛋白的小鼠血清中组胺和免疫球蛋白IgE浓度均高于注射PBS的阴性对照组,表明这2种金小蜂类icarapin蛋白有潜在的致敏风险。本研究为寄生蜂源活性蛋白在应用前的安全性评估提供了一定的参考。The icarapin protein of Western honey bee,Apis mellifera,is a common human allergen and its homologues also exist in parasitoid wasps.In order to evaluate the potential allergenicity of icarapin-like proteins in parasitoid wasps,the icarapin-like protein encoding genes from Pteromalus puparum and Nasonia vitripennis were successfully cloned and expressed.Sequence analysis showed that the open reading frames of icarapin-like genes in the two parasitoid wasps were 654 bp and 714 bp,respectively,and both were predicted to be potentially allergenic.The recombinant icarapin-like proteins were purified by Ni-ion affinity chromatography.Using the BALB/c mouse model for potential allergenicity assessment,we found that the concentrations of histamine and immunoglobulin E(IgE)in mice serum injected with recombinant proteins of the two parasitoid wasps and A.mellifera were higher than those of the negative control group injected with PBS,indicating that icarapin-like proteins from the two pteromalid wasps have potential allergenicity.This study provides a reference for the pre-application safety assessment of parasitoid bioactive proteins.

关 键 词：蝶蛹金小蜂 丽蝇蛹集金小蜂 icarapin蛋白 表达与纯化 致敏性评价 

分 类 号：S476.3[农业科学—农业昆虫与害虫防治]