防治玉米土传病和地下害虫蛴螬复合微生物种衣剂的研制  

Development of Compound Microbial Seed Coating Agent for the Control of Soil-borne Diseases and Underground Pest Protaetia brevitarsis in Maize Fields

作　　者：王江平 王雅娜 张飞燕[2] 殷嘉璐 刘洪伟[2] 董尧坤 刘秋玥 张丽萍[2] WANG Jiangping;WANG Yana;ZHANG Feiyan;YIN Jialu;LIU Hongwei;DONG Yaokun;LIU Qiuyue;ZHANG Liping(College of Life Sciences,Hebei Normal University,Shijiazhuang 050024,China;Institute of Biology,Hebei Academy of Science,Shijiazhuang 050081,China;School of Life Sciences and Engineering,Hebei University of Science and Technology,Shijiazhuang 050018,China)

机构地区：[1]河北师范大学生命科学学院,石家庄050024 [2]河北省科学院生物研究所,石家庄050081 [3]河北科技大学生命科学与工程学院,石家庄050018

出　　处：《中国生物防治学报》2023年第5期1124-1132,共9页Chinese Journal of Biological Control

基　　金：河北省科学院科技计划项目(2022305)。

摘　　要：针对目前微生物源杀虫型种衣剂严重缺乏的现状,本文以3株具有杀虫防病功能的芽胞杆菌及其代谢产物为主要成分,研制一种杀虫防病复合微生物种衣剂。通过测定助剂的理化性质及其对3株芽胞杆菌生长的影响,确定助剂种类和用量,最终形成生物种衣剂的配方:贝莱斯芽胞杆菌ZLP-101(≥2×10^(9)cfu/mL)、解淀粉芽胞杆菌ZLP-01(≥2×10^(8)cfu/mL)和枯草芽胞杆菌ZLP-121(1×10^(8)cfu/mL)以1:1:1体积比混合,辅以成膜剂海藻酸钠3%、化学增效剂高效氯氟氰菊酯0.01%、着色剂酸性品红0.2%、增稠剂黄原胶0.1%、分散剂木质素磺酸钠2%。以玉米为供试作物,室内盆栽试验结果显示,该生物种衣剂对地下害虫蛴螬的防效为70.71%,对玉米立枯病的防效达83.64%。研制的种衣剂的相关指标符合悬浮种衣剂及微生物菌剂的国家标准,可用于玉米包衣,防治土传病和地下虫害。Microbial insecticidal seed coating agents are not available.This study aims to develop an insecticidal and disease-preventing compound microbial seed coating agent with three strains of bacillus with insecticidal and disease-preventing functions and their metabolites as the main components.The type and dosage of the auxiliaries for the seed coating agent were determined through analyses of the physicochemical properties of various additives and their influence on the growth of the 3 strains of Bacillus,which was formulated as:B.velezensis ZLP-101(≥2×10^(9) cfu/mL),B.amyloliquefaciens ZLP-01(≥2×10^(8) cfu/mL),and B.subtilis ZLP-121(1×10^(8) cfu/mL)mixed in a volume ratio of 1:1:1,supplemented by the film-forming agent sodium alginate 3%,the chemical synergist high efficiency cypermethrin 0.01%,the colorant acid magenta 0.2%,the thickener xanthan gum 0.1%,and the dispersant sodium lignosulfonate 2%.The agent was tested using potted maize plants in the laboratory,which showed that the efficacy of the biological seed coating agent against underground pest grubs was 70.71%,and the control efficacy of maize blight was 83.64%.The relevant indicators of this coating agent comply with the national standards for suspension seed coating agents and microbial fungal agents,which can be used for corn coating,control of soil-borne diseases and underground insect pests,and has great application potential in seed coating of other crops.

关 键 词：芽胞杆菌 立枯丝核菌 微生物种衣剂 

分 类 号：S476[农业科学—农业昆虫与害虫防治]