青柏散对口腔溃疡模型大鼠的药效学研究  

Pharmacodynamics of Qingbai pulvis on oral ulcer in rat models

作　　者：赵慧雯 杨芮庚 呼延铭 赵锐帆 雷丸 潘雯 张晓莉 王济 冯斌 ZHAO Hui-wen;YANG Rui-geng;HU Yan-ming;ZHAO Rui-fan;LEI Wan;PAN Wen;ZHANG Xiao-li;WANG Ji;FENG Bin(State Key Laboratory of Military Stomatology&National Clinical Research Center for Oral Diseases&Shaanxi Engineering Research Center for Dental Materials and Advanced Manufacture,Department of Pharmacy,School of Stomatology,The Fourth Military Medical University,Xi’an 710032)

机构地区：[1]军事口腔医学国家重点实验室,国家口腔疾病临床医学研究中心,陕西省口腔生物工程技术研究中心,第四军医大学口腔医院药剂科,西安710032

出　　处：《中南药学》2023年第11期2957-2963,共7页Central South Pharmacy

基　　金：陕西省中医药管理局科研项目(No.2021-ZZ-ZY001)。

摘　　要：目的探讨青柏散对口腔溃疡模型大鼠的药效学作用。方法将126只雄性SD大鼠随机均分为正常组,模型组,阳性药组,青柏散低、中、高剂量组(0.25、0.50、0.75 g·kg^(-1))。每组取18只采用冰醋酸化学灼烧法建立口腔溃疡实验模型,各药物组分别散布相应量药粉,连续给药4 d,模型组和正常组不做任何处理。观察各组大鼠体质量变化以及创面愈合情况,苏木素-伊红(HE)染色法观察溃疡组织病理变化,酶联免疫吸附测定法(ELISA)检测碱性成纤维细胞生长子(bFGF)、白细胞介素(IL)-2、IL-6和肿瘤坏死因子-α(TNF-α)的含量。另每组取3只大鼠采用稳定开口法建立疼痛实验模型,Von Frey法测定机械疼痛阈值。结果与模型组相比,给药组大鼠体质量下降的情况显著改善(P<0.05);口腔溃疡区黏膜皮层明显修复,溃疡部位炎性细胞浸润明显减少;炎性因子bFGF和IL-2的含量显著增加(P<0.05),IL-6和TNF-α的含量显著下降(P<0.05)。给药处理使大鼠口腔溃疡区机械疼痛阈值显著提高(P<0.05)。结论青柏散可明显加快大鼠口腔溃疡的愈合,减轻炎症反应并缓解疼痛程度。Objective To determine the pharmacodynamics of Qingbai pulvis on oral ulcer in rat models.Methods Totally 126 male SD rats were randomly divided into a normal group,a model group,a positive group,and Qingbai pulvis low-dose,medium-dose and high-dose groups(0.25,0.50,and 0.75 g·kg^(-1)).Eighteen rats in each group were used to induce the oral ulcer model by chemical burning with acetic acid.The administration groups were given corresponding medicine for 4 consecutive days,while the normal group and the model group had no treatment.The weight change and wound healing were observed.Hematoxylin-eosin(HE)staining method was used to observe the histopathological changes of oral ulcer,and enzyme-linked immunosorbent assay(ELISA)method was used to detect the content of basic fibroblast growth factor(bFGF),interleukin(IL)-2,IL-6 and tumor necrosis factor-α(TNF-α)in the ulcer tissues of rats.Another 3 rats in each group were used to induce a pain model by stable mouth opening method.Von Frey method was used to measure pain threshold.Results Compared with the model group,the weight loss was improved in the administration groups(P<0.05).The mucosal cortex was greatly repaired and the infiltration of inflammatory cells at the ulcer was obviously reduced.The content of bFGF and IL-2 was significantly increased(P<0.05),and the content of IL-6 and TNF-αwas decreased(P<0.05).The mechanical pain threshold of rat oral ulcer was significantly increased after the treatment(P<0.05).Conclusion Qingbai pulvis can accelerate the healing of rat oral ulcer,reduce inflammation and relieve pain.

关 键 词：青柏散 口腔溃疡 药效学作用 

分 类 号：R285[医药卫生—中药学]