拮抗菌BJB01抗黄萎病的抗病效果评价  

Evaluation of the Anti-disease Effect of Antagonistic Bacterium BJB01 Against Verticillium wilt

作　　者：段平成 郑凯[1] 张宇宏[2] 张国丽[3] 孙国清[2] DUAN Pingcheng;ZHENG Kai;ZHANG Yuhong;ZHANG Guoli;SUN Guoqing(Cotton Engineering Research Center,College of Agriculture,Xinjiang Agricultural University,Ministry of Education,Urumqi 830052,China;Institute of Biotechnology,Chinese Academy of Agricultural Sciences,Beijing 100081,China;Xinjiang Academy of Agricultural Reclamation,Xinjiang Shihezi 832000,China)

机构地区：[1]新疆农业大学农学院,棉花教育部工程研究中心,乌鲁木齐830052 [2]中国农业科学院生物技术研究所,北京100081 [3]新疆农垦科学院,新疆石河子832000

出　　处：《生物技术进展》2023年第6期913-918,共6页Current Biotechnology

基　　金：国家自然科学基金项目(31771851)。

摘　　要：棉花是重要的战略与民生物资,在种植过程中面临多种不利因素的影响,其中棉花黄萎病作为一种危害性极大的植物性疾病使棉花大量减产,因此需要一种安全高效的方法应对棉花黄萎病的发生。从新疆棉花重病田中筛选到菌株BJB01并进行提取纯化,通过生理生化鉴定和革兰氏染色试验,对获得的菌株DNA进行16S rDNA测序,将测序结果在NCBI上进行比对;然后利用平板对峙试验初步测试防效;最后通过温室防效试验,筛选到最佳的防治方法及浓度。试验结果显示,该菌与贝莱斯芽孢杆菌相似度极高,为99.71%,由此可确定该菌为贝莱斯芽孢杆菌(Bacillus belleiensis);在平板对峙中,该菌显现出对大丽轮枝菌(Verticillium dahlia Kleb.)的抑制作用;通过温室防效测试发现该菌在浓度为OD600=0.6时的防效结果达到89.35%,优于其他方法及处理浓度,并且再次单独测试后防效为85.91%,效果依旧最佳。综合分析,从新疆棉花重病田中获得的贝莱斯芽孢杆菌BJB01,可以对大丽轮枝菌起抑制作用,有望用于制备生物菌剂用以棉花生产防治。Cotton is an important strategic and livelihood material,which faces many unfavorable factors in the cultivation process,among which cotton Verticillium wilt,as a very harmful plant disease,could cause a large reduction in cotton production.Therefore,a safe and efficient method is needed to deal with the occurrence of cotton Verticillium wilt.We extracted and purified the strain BJB01 from a severely diseased cotton field in Xinjiang.Physiological and biochemical identification and Gram staining test were performed.And we also extracted the DNA of the strain for 16S rDNA sequencing,and blasted the sequencing results on NCBI;then we used the plate standoff test to initially test the effectiveness of the prevention and control;finally,we screened the best prevention and control method and concentration through the greenhouse prevention and control test.Through the test results,the bacterium was found to be 99.71%similar to Bacillus belleiensis,which can be identified as B.belleiensis;and the bacterium showed inhibition of Verticillium dahlia Kleb.in the plate standoff test;through the greenhouse efficacy test,it was found that the bacterium at the concentration of OD600=0.6 had the preventive effect of 89.35%,which was better than other methods and treatment concentrations,and the preventive effect of 85.91%was still the best effect in the separate test again.In the comprehensive analysis,Bacillus pelliculatus BJB01 obtained from the cotton disease field in Xinjiang can inhibit Verticillium dahliae,which could be prepared as a biofungicide for cotton production.

关 键 词：棉花 拮抗菌 黄萎病 大丽轮枝菌 贝莱斯芽孢杆菌 

分 类 号：Q939.95[生物学—微生物学] S562[农业科学—作物学]