氢分子对咪喹莫特诱导的小鼠银屑病样皮损的抑制作用  

Inhibitory Effect of Hydrogen Molecule on Imiquimod-induced Psoriasis-like Dermatitis in Mice

作　　者：朱钧锴 葛玲智 张超 曹璨 吴嘉惠 穆震 ZHU Junkai;GE Lingzhi;ZHANG Chao;CAO Can;WU Jiahui;MU Zhen(Dermatology Department,Tai’an 88 Hospital,Shandong Tai’an 271000,China;Dermatology Department,the Second Affiliated Hospital of Shandong First Medical University,Shandong Tai’an 271000,China)

机构地区：[1]泰安八十八医院皮肤科,山东泰安271000 [2]山东第一医科大学第二附属医院皮肤科,山东泰安271000

出　　处：《生物技术进展》2023年第6期945-953,共9页Current Biotechnology

基　　金：泰安市科技创新发展项目(2020NS100)。

摘　　要：氢分子是一种新型的气体信号分子,对多种炎症疾病有一定的作用效果。银屑病是一种慢性炎症性疾病,氢分子有望作为银屑病辅助治疗的新手段。连续7 d使用5%咪喹莫特(imiquimod,IMQ)乳膏诱导小鼠银屑病模型,期间每天2次、每次2 h将小鼠置于35%的氢分子环境中进行干预。每天观察小鼠皮损变化并进行银屑病面积和严重程度指数(psoriasis area and severity index,PASI)评分;第8天,处死小鼠取皮损,苏木精-伊红(hematoxylin-eosin,HE)染色并使用Baker评分评估组织病理学损害程度;免疫组化检测皮损中炎症因子IL-6、TNF-α和IFN-γ的表达;实时荧光定量PCR(real-time quantitative fluorescent polymerase chain reaction,RT-qPCR)和蛋白免疫印迹检测皮损中炎症因子IL-6、TNF-α和IFN-γ的相对mRNA水平和蛋白水平的表达;用试剂盒检测皮损组织中氧化指标丙二醛(malondialdehyde,MDA)含量和抗氧化相关的过氧化氢酶(catalase,CAT)活力。结果显示,氢分子干预降低了IMQ诱导小鼠的PASI评分(P<0.05)和Baker评分(P<0.001),改善了组织的病理学变化;氢分子干预组小鼠皮损组织中IL-6、TNF-α和IFN-γ的mRNA及蛋白表达水平均下降,MDA的含量下降,CAT的活力增加(P<0.05)。结果表明,氢分子可抑制银屑病样皮损的形成、减轻炎症反应、减轻氧化应激损伤,有望成为治疗和缓解银屑病的新方法。Hydrogen molecule is a new type of gas signaling molecule,which has a certain effect on a variety of inflammation diseases.Psoriasis is a chronic,inflammatory disease,and molecular hydrogen is expected to be a new adjuvant therapy for psoriasis.The psoriasis model of mice was induced by 5% imquimod(IMQ)cream for 7 consecutive days,during which the mice were exposed to 35% hydrogen molecular environment twice a day for 2 h each time for intervention.The changes of skin lesions were observed daily and the psoriasis area and severity index(PASI)score was performed.On the 8th day,the skin lesions were taken by death,hematoxylin-eosin(HE)staining,and the histopathological lesion was evaluated using Baker score.The expressions of inflammatory cytokines IL-6,TNF-α and IFN-γ were detected by immunohistochemistry.Real-time quantitative fluorescent PCR(RT-qPCR)and Western blot were used to detect the relative mRNA and protein levels of inflammatory cytokines IL-6,TNF-α and IFN-γ in skin lesions.The content of oxidation index malondialdehyde(MDA)and the activity of antioxidation-related catalase(CAT)were detected by the kit.Results showed that,molecular hydrogen intervention decreased PASI score(P<0.05)and Baker score(P<0.001),and improved histopathological changes of IMQ-induced mice.The mRNA and protein expression levels of IL-6,TNF-α and IFN-γ in the skin tissue of mice in the hydrogen molecular intervention group were decreased,the content of MDA was decreased,and the activity of CAT was increased(all P<0.05).The results indicated that,molecular hydrogen can inhibit the formation of psoriasis skin lesions,reduce inflammation and oxidative stress damage,which has the potential to treat psoriasis.

关 键 词：氢分子 银屑病样皮损 氧化应激 炎症反应 

分 类 号：Q819[生物学—生物工程] R758.63[医药卫生—皮肤病学与性病学]