微藻曝气强化短程硝化/厌氧氨氧化自养脱氮工艺性能  

Performance of autotrophic denitrification enhanced by microalgae aeration and anaerobic ammox

作　　者：刘春爽[1] 张璐遥 尹海[3] 李世文 王明艳 刘力嘉 王胜渊 孙志超 LIU Chunshuang;ZHANG Luyao;YIN Hai;LI Shiwen;WANG Mingyan;LIU Lijia;WANG Shengyuan;SUN Zhichao(College of Chemistry and Chemical Engineering in China University of Petroleum(East China),Qingdao 266580,China;Judicial Bureau of Pingyi County,Linyi 273399,China;Fengcheng Oilfield Operation Area of Xinjiang Oilfield Company,PetroChina,Karamay 834000,China;Qingdao West Coast Utility Group Water Company,Qingdao 266000,China)

机构地区：[1]中国石油大学(华东)化学化工学院,山东青岛266580 [2]山东省临沂市平邑县司法局,山东临沂273399 [3]中国石油新疆油田公司风城油田作业区,新疆克拉玛依834000 [4]青岛西海岸新区公用事业集团水务有限公司,山东青岛266000

出　　处：《中国石油大学学报（自然科学版）》2023年第6期185-191,共7页Journal of China University of Petroleum(Edition of Natural Science)

基　　金：山东省自然科学基金项目(ZR2019MEE038);中央高校科研业务费专项(19CX02038A);2022年青岛西海岸新区科技计划项目科技攻关“揭榜制”专项。

摘　　要：采用序批式生物膜反应器(SBR),在温度为30℃条件下,在短程硝化/厌氧氨氧化生物膜的基础上耦合小球藻构建藻菌耦合生物膜体系,通过改变光照时长和曝气量组合条件的运行方式共运行85 d,分析组合条件下体系的脱氮性能、藻菌耦合体系特性和氮转化路径,以得到最佳脱氮条件及藻菌耦合脱氮机制。结果表明:当进水氨氮质量浓度为(400±50) mg/L时,光暗比(单位为h/h)设置为6/4,曝气强度为200 mL/min时,脱氮效果最好,NH4+-N及总氮平均去除率最高可达92.31%和87.56%;藻菌耦合体系运行过程中污泥质量浓度与小球藻干重calgae比始终约为5.5,表明生物膜中藻类和细菌的比例达到相对稳定状态,并形成良好的互利共生关系,集中在生物膜外部的小球藻通过光合作用产生的氧气被硝化细菌消耗,因此产生的厌氧环境和亚硝酸盐底物来维持厌氧氨氧化菌的活性;在氮的去除机制中,生物吸收量约占45.71%,PN/A(短程硝化-厌氧氨氧化)过程N2的生成量及氮损失约占54.29%。A sequencing batch biofilm(SBR)reactor was used to cultivate an algae-coupled biofilm system based on short-range nitrification/anaerobic ammonia oxidation biofilm with Chlorella vulgaris at a temperature of 30℃.The system was op-erated for 85 d by changing the combination of light duration and aeration.The nitrogen removal performance,algae-coupled system characteristics and nitrogen conversion pathways were analyzed to obtain the optimal nitrogen removal conditions and algal-bacterial coupling nitrogen removal mechanism.The results show that when the influent ammonia nitrogen concentration is 400±50 mg/L,the light/dark ratio is 6 h/4 h,and the aeration intensity is 200 mL/min,the best denitrification effect was achieved,in which the average removal rates of NH4+-N and total nitrogen were up to 92.31%and 87.56%,respective-ly.The ratio of cMLVSS(wastewater mass concentration)to calgae(dry weight of chlorella vulgaris)is always around 5.5 during the operation of the algae coupled system,which indicates that the ratio of algae and bacteria in the biofilm reaches a relative-ly stable state and forms a good mutually beneficial symbiotic relationship.The oxygen produced by the photosynthesis of Chlorella vulgaris concentrated outside the biofilm is consumed by nitrifying bacteria,and nitrifying bacteria provided anaero-bic environment and nitrite substrate to maintain the activity of anaerobic ammonia oxidizing bacteria.In the nitrogen removal mechanism,the biological uptake accounts for about 45.71%,and the amount of N2 production and nitrogen loss in the PN/A(partial nitrification-anaerobic ammonium oxidation)process accounts for about 54.29%.

关 键 词：小球藻 藻菌耦合体系 短程硝化/厌氧氨氧化 生物膜 自养脱氮 

分 类 号：X522[环境科学与工程—环境工程]