人工胃/肠液和大鼠肠道菌群对氟苯尼考磺胺噻唑稳定性的影响  

Effects of Artificial Gastric/Intestinal Fluid and Intestinal Flora on Stability of Florfenicol Sulfathiazole

作　　者：朱兆晗 刘希望[2] 杨亚军[2] 白莉霞 秦哲[2] 李世宏[2] 李准 李剑勇[1,2] 葛闻博 ZHU Zhao-han;LIU Xi-wang;YANG Ya-jun;BAI Li-xia;QIN Zhe;LI Shi-hong;LI Zhun;LI Jian-yong;GE Wen-bo(College of Veterinary Medicine,Gansu Agricultural University,Lanzhou,Gansu,730070,China;Lanzhou Institute of Animal Husbandry and Pharmaceutical Science,Chinese Academy of Agricultural Sciences/Key Laboratory of New Animal Drug Discovery,Ministry of Agriculture and Rural Affairs/Key Laboratory of New Animal Drug Project of Gansu Province,Lanzhou,Gansu,730050,China)

机构地区：[1]甘肃农业大学动物医学院,甘肃兰州730070 [2]中国农业科学院兰州畜牧与兽药研究所/农业农村部兽用药物创制重点实验室/甘肃省新兽药工程重点实验室,甘肃兰州730050

出　　处：《动物医学进展》2023年第12期76-81,共6页Progress In Veterinary Medicine

基　　金：中央公益性科研院所基本科研业务费专项资金项目(1610322022012)。

摘　　要：为新型偶联化合物-氟苯尼考磺胺噻唑(FST)研制和开发口服制剂提供数据支持,采用高效液相色谱法,对FST在5种溶液介质(无蛋白酶人工肠液、人工肠液、无蛋白酶人工胃液、人工胃液及大鼠肠道菌群培养液)中不同时刻的含量进行了研究,判断其稳定性差异。结果表明,FST在人工胃液和无蛋白酶人工胃液0~6 h含量保持基本稳定;在人工肠液和无蛋白酶人工肠液中孵育30 min已完全降解,且新出现的色谱峰在相同色谱条件下与氟苯尼考和磺胺噻唑对照品出峰时间相同。由此可知,FST的代谢产物为氟苯尼考和磺胺噻唑;FST在肠道菌群中发生了降解,0~8 h,FST含量迅速下降,而8~12 h趋于平稳,降至50%。说明FST在人工肠液中有明显的降解,在人工胃液中趋于稳定,肠道菌群对其稳定性也有一定的影响。To provide data support for the development of oral preparations for the novel coupling compound florfenicol sulfathiazole(FST),high performance liquid chromatography was used to determine FST in five solution media:artificial intestinal fluid without protease,artificial intestinal fluid,artificial gastric juice without protease,artificial gastric juice,and the content of intestinal flora culture medium of rats at different times were studied to determine the difference in their stability.The results showed that the content of FST remained basically stable in 0-6 h of artificial gastric juice and artificial gastric juice without protease;After incubation in artificial intestinal fluid and artificial intestinal fluid without protease for 30 min,the chromatographic peak was completely degraded,and the emerging chromatographic peak time was the same as that florfenicol and sulfathiazole reference substance under the same chromatographic conditions.It was concluded that the metabolites of FST were florfenicol and sulfathiazole;FST degradation occurred in the intestinal flora.The content of FST decreased rapidly at 0-8 h,and stabilized to 50%at 8-12 h.These results indicated that FST is obviously degraded in artificial intestinal fluid and tends to be stable in artificial gastric juice,and the intestinal flora also has a certain influence on its stability.

关 键 词：氟苯尼考磺胺噻唑 人工肠液 人工胃液 肠道菌群 稳定性 

分 类 号：S859.7[农业科学—临床兽医学]