Fuchs综合征并发白内障患者房水中细胞因子及病毒学检测观察  

Observation on the detection of cytokines and virus in aqueous humor of patients with Fuchs syndrome complicated with cataract

作　　者：张曙光[1] 王慧芳 项杰 王瑞峰[1] 赵宏[1] ZHANG Shuguang;WANG Huifang;XIANG Jie;WANG Ruifeng;ZHAO Hong(Department of Ophthalmology,Zhengzhou Second People’s Hospital,Zhengzhou 450006,Henan Province,China;Department of Ophthalmology,Henan Provincial the Third People’s Hospital,Zhengzhou 450000,Henan Province,China)

机构地区：[1]郑州市第二人民医院眼科,河南省郑州市450006 [2]河南省直第三人民医院眼科,河南省郑州市450000

出　　处：《眼科新进展》2023年第12期987-991,共5页Recent Advances in Ophthalmology

摘　　要：目的 检测Fuchs综合征并发白内障患者房水中细胞因子[白细胞介素-6(IL-6)、白细胞介素-8(IL-8)、白细胞介素-10(IL-10)、血管内皮生长因子(VEGF)、细胞黏附分子(CAM)、碱性成纤维细胞生长因子(BFGF)]、巨细胞病毒(CMV)核酸、风疹病毒(RV)核酸及病毒抗体的表达水平,评价Fuchs综合征患者房水中炎症及病毒表达情况。方法 回顾性研究。选取郑州市第二人民医院眼科2020年10月至2022年7月临床诊断为Fuchs综合征并发白内障患者18例(18眼)纳入研究。记录患者术前一般资料,Fuchs综合征并发白内障患者均行超声乳化白内障吸除及人工晶状体植入术,术中均抽取房水0.1 mL。定量分析房水中细胞因子(IL-6、IL-8、IL-10、VEGF、CAM、BFGF)、CMV核酸、RV核酸及病毒抗体表达水平。结果 18例Fuchs综合征患者检测了房水中IL-6水平,其中13例正常,5例轻度升高;8例患者检测了IL-8、IL-10、VEGF、CAM、BFGF水平,其中IL-10水平均正常,IL-8水平有2例升高,VEGF水平有1例轻度升高,CAM及BFGF水平各有7例升高,其余均正常。12例患者检测了CMV及RV核酸,结果均为阴性。参与检测的18例患者中,12例RV抗体(RV-IgG)升高,6例正常,但均有一定滴度;16例患者检测了CMV抗体(CMV-IgG)及单纯疱疹病毒抗体,仅1例患者CMV-IgG轻度升高,其余均正常;8例患者检测了水痘-带状疱疹病毒抗体表达,均为阴性。结论 Fuchs综合征并发白内障患者眼内炎症活动不明显,临床RV核酸不容易被检测到,而RV-IgG对临床诊断Fuchs综合征有一定的参考价值。Objective To detect the expression levels of cytokines[Interleukin-6(IL-6),Interleukin-8(IL-8),Interleukin-10(IL-10),vascular endothelial growth factor(VEGF),cell adhesion molecule(CAM)and basic fibroblast growth factor(BFGF)],cytomegalovirus(CMV)nucleic acid,rubella virus(RV)nucleic acid and antibodies in patients with Fuchs syndrome and cataract,evaluating the inflammation and viral expression in aqueous humor of patients.Methods A retrospective study was conducted.A total of 18 patients(18 eyes)clinically diagnosed with Fuchs syndrome complicated with cataract from October 2020 to July 2022 in the Ophthalmology Department of the Zhengzhou Second Hospital were included and their preoperative data were recorded.These patients underwent phacoemulsification and intraocular lens implantation,with 0.1 mL aqueous humor extracted during the operation.The expression levels of cytokines(IL-6,IL-8,IL-10,VEGF,CAM and BFGF),CMV nucleic acid,RV nucleic acid and viral antibodies were quantitatively analyzed.Results In these 18 patients,the IL-6 level was normal in 13 patients and mildly elevated in 5 patients.The levels of IL-8,IL-10,VEGF,CAM and BFGF were detected in 8 patients,IL-10 was normal in all these patients,IL-8 was elevated in 2 patients,VEGF was mildly elevated in 1 patient,and CAM and BFGF levels were increased in 7 patients.The CMV and RV nucleic acids were tested in 12 patients,with all negative results.In the 18 patients involved,the RV-IgG increased in 12 patients and was normal in other 6 patients with certain titer values.In 16 patients tested for CMV-IgG and herpes simplex virus-IgG,only one patient was slightly elevated in CMV-IgG,and the rest were normal.Varicella-zoster virus-IgG was tested in 8 patients,and all of them were negative.Conclusion The eye inflammation of patients with Fuchs syndrome and cataract is not obvious,thus clinical RV nucleic acid is not easily detected,and the RV-IgG has a reference value in clinical diagnosis of Fuchs syndrome.

关 键 词：FUCHS综合征 白内障 眼内液 细胞因子 风疹病毒 巨细胞病毒 

分 类 号：R773.3[医药卫生—眼科]