植物源抗真菌肽原材料的筛选及桂皮抗菌肽提取工艺的优化  

Screening of Plant-Derived Antifungal Peptides and Optimization of Extraction Process of Antifungal Peptides from Cinnamon

作　　者：王虹霁 赵展展 李梦圆 张超文 王敬仪 韩玉竹[1,2] WANG Hongji;ZHAO Zhanzhan;LI Mengyuan;ZHANG Chaowen;WANG Jingyi;HAN Yuzhu(College of Animal Science and Technology,Rongchang Chongqing 402460,China;Immunology Research Center,Medical Research Institute,Southwest University,Rongchang Chongqing 402460,China)

机构地区：[1]西南大学动物科学技术学院,重庆荣昌402460 [2]西南大学医学院免疫学中心,重庆荣昌402460

出　　处：《西南大学学报(自然科学版)》2023年第12期76-84,共9页Journal of Southwest University(Natural Science Edition)

基　　金：国家自然科学青年基金项目(31901929);重庆市自然科学基金面上项目(cstc2021jcyj-msxmX1021);中央高校基本业务费专项资金重点项目(XDJK2020B015).

摘　　要：为寻找抑制霉菌的植物源抗真菌肽(AMPs)的原材料,选择了53种具有潜在抑菌特性的植物材料,以黄曲霉和黑曲霉为指示菌,用硫酸铵沉淀法提取抗菌肽,对其抑菌活性进行评定;进一步选取桂皮为原材料,从浸提液种类、料液比、浸提时间和硫酸铵饱和度4个方面进行抗菌肽提取工艺的优化.结果表明:75.47%的供试样材料含有抗菌肽,籽实及根茎类含抗菌肽的比例为86.11%,明显高于花叶类(50%).抑菌圈直径达到15 mm以上的包括大蒜、丁香、桂皮、辣椒籽、桑叶、白芨、木香等12种材料.对桂皮提取工艺进行优化,得出在浸提液为PBS,料液比为1∶5.11 g/mL,浸提时间为39.29 h,硫酸铵饱和度为81.74%的条件下抑菌效果最好,抑菌圈直径可达到33.74 mm(p<0.05).研究结果显示:抗菌肽广泛存在于植物组织中;植物本身可利用的部位中,籽实及根茎是抗菌肽分布的主要部位;提取工艺优化后,桂皮抗菌肽抑菌效果提高了21.41%.To find the raw materials of plant-derived antifungal peptides for inhibiting mold,53 plant materials with potential antifungal peptides were selected for their antifungal activities against Aspergillus flavus and Aspergillus niger determined by inhibition zone diameter.The antifungal peptides were extracted with ammonium sulfate precipitation method.Cinnamon was further selected as the raw material to optimize the extraction process of antifungal peptides from four aspects:the type of extraction solution,the ratio of solid to liquid,the extraction time and the saturation of ammonium sulfate.The results showed that 75.47%of the selected 53 samples contained antifungal peptides.The proportion of seeds and rhizomes containing antifungal peptides was 86.11%,which was significantly higher than that of flowers and leaves(50%).There were 12 materials with an antifungal zone diameter more than 15 mm,including garlic,clove,cinnamon,pepper seeds,mulberry leaves,Bletilla striata and Aucklandiae radix.The extraction process of cinnamon was optimized.The results showed that the antifungal activity was the best when the extraction solution was PBS,the solid-liquid ratio was 1∶5.11 g/mL,the extraction time was 39.29 h and the saturation of ammonium sulfate was 80.74%,with an antifungal zone diameter of 33.74 mm(p<0.05).Antifungal peptides widely exist in plant tissues.Among the available parts of plants,seeds and rhizomes were the main parts of the distribution of antifungal peptides.After optimizing the extraction process,the antifungal activity of cinnamon antifungal peptide was increased by 21.41%.

关 键 词：植物源抗真菌肽 生物防霉 桂皮 AMPs提取优化 

分 类 号：Q946.8[生物学—植物学]