血清Apelin-13和网膜素-1表达与肾移植术后患者肾功能的相关性研究  

Correlations of serum Apelin-13 and Omentin-1 expression with renal function in patients after renal transplantation

作　　者：李冰玉[1] 师勇[1] 陈家会 梁亚林[1] 唐倩 杨青彦[1] LI Bingyu;SHI Yong;CHEN Jiahui;LIANG Yalin;TANG Qian;YANG Qingyan(Kidney Transplant Kidney Disease Diagnosis and Treatment Center,the Seventh People′s Hospital of Zhengzhou in Henan Province,Zhengzhou,Henan,450000)

机构地区：[1]河南省郑州市第七人民医院肾移植肾脏病诊疗中心,河南郑州450000

出　　处：《实用临床医药杂志》2023年第20期6-10,15,共6页Journal of Clinical Medicine in Practice

基　　金：河南省医学科技攻关计划联合共建项目(LHGJ20210756)。

摘　　要：目的探讨血清Apelin-13、网膜素-1(Omentin-1)在早期预测肾移植术后并发急性肾功能损伤中的价值。方法纳入89例肾移植患者为肾移植组,根据肾移植术后1个月内患者是否发生急性肾功能损伤分为肾功能损伤组21例和非肾功能损伤组68例。同时随机选取健康体检者87例作为对照组。采用酶联免疫吸附(ELISA)法检测血清Apelin-13、Omentin-1水平;采用粒子增强透射免疫比浊法测定胱抑素C水平;采用酶法测定血肌酐水平;采用紫外-谷氨酸脱氨酶法测定血尿素氮水平;采用比浊法测定β2-微球蛋白、24 h尿蛋白水平。校正eMDRD方程,计算估算肾小球滤过率(eGFR)。采用Pearson法进行术后肾功能损伤的肾移植患者血清Apelin-13、Omentin-1水平与肾功能指标的相关性分析;采用受试者工作特征(ROC)曲线分析血清Apelin-13、Omentin-1水平对肾移植患者术后肾功能损伤的预测价值;采用Logistic回归分析法分析肾移植患者术后肾功能损伤的影响因素。结果肾移植组的血肌酐、血尿素氮、胱抑素C、β2-微球蛋白、24 h尿蛋白水平高于对照组,Apelin-13、Omentin-1、eGFR水平低于对照组,差异有统计学意义(P<0.05)。肾功能损伤组的血肌酐、血尿素氮、胱抑素C、β2-微球蛋白、24 h尿蛋白水平高于非肾功能损伤组,Apelin-13、Omentin-1、eGFR水平低于非肾功能损伤组,差异有统计学意义(P<0.05)。术后肾功能损伤的肾移植患者血清Apelin-13、Omentin-1水平与肌酐、血尿素氮、胱抑素C、β2-微球蛋白、24 h尿蛋白呈负相关,与eGFR呈正相关(P<0.05)。Apelin-13、Omentin-1单独及联合预测肾移植患者术后肾功能损伤的曲线下面积(AUC)分别为0.784、0.773、0.841。Apelin-13、Omentin-1是肾移植患者术后肾功能损伤的保护因素(P<0.05)。结论肾移植术后发生肾功能损伤患者血清中Apelin-13、Omentin-1均呈低表达,其或可成为早期预测指标。Objective To investigate the value of serum Apelin-13 and Omentin-1 in early prediction of acute renal injury after renal transplantation.Methods A total of 89 renal transplantation patients were included in the renal transplantation group,and were divided into renal injury group(21 cases)and non-renal injury group(68 cases)according to whether acute renal injury occurred within one month after renal transplantation.At the same time,87 healthy people were randomly selected as the control group.Serum Apelin-13 and Omentin-1 levels were detected by enzyme-linked immunosorbent assay(ELISA);the Cystatin C levels were determined by particle enhanced transmission immunoturbidimetry;serum creatinine level was determined by enzyme method;the blood urea nitrogen level was determined by UV-glutamate deaminase method;the levels ofβ2-microglobulin and 24 h urinary protein were measured by turbidimetry.The eMDRD equations were corrected and estimated glomerular filtration rate(eGFR)was calculated.Pearson method was used to analyze the correlations of serum Apelin-13 and Omentin-1 levels with renal function indexes in renal transplantation patients with postoperative renal function injury;receiver operating characteristic(ROC)curve was used to analyze the predictive value of serum Apelin-13 and Omentin-1 levels on postoperative renal function injury in renal transplantation patients;Logistic regression analysis was used to analyze the influencing factors of renal function injury after renal transplantation.Results The levels of serum creatinine,blood urea nitrogen,cystatin C,β2-microglobulin and 24 h urinary protein in the renal transplantation group were significantly higher,and the levels of Apelin-13,Omentin-1 and eGFR were significantly lower than those in the control group(P<0.05).The levels of serum creatinine,blood urea nitrogen,cystatin C,β2-microglobulin and 24 h urinary protein in the renal injury group were significantly higher,and the levels of Apelin-13,Omentin-1 and eGFR were significantly lower than those in

关 键 词：肾移植 APELIN-13 网膜素-1 肾功能损伤 

分 类 号：R692[医药卫生—泌尿科学] R446[医药卫生—外科学]