糖耐康改善KKAy小鼠Toll样受体4通路及葡萄糖转运子4的作用机制研究  

Mechanism of Tangnai Kang in Improving TLR4 Signaling Pathway and GluT-4 in KKAy Diabetic Mice

作　　者：王芬 何华亮[2] 刘铜华[3] 吴丽丽[3] WANG Fen;HE Hualiang;LIU Tonghua;WU Lili(Beijing University of Chinese Medicine Third Affiliated Hospital,Beijing 100029,China;Beijing General Hospital of the Chinese People′s Armed Police Force,Beijing 100027,China;Beijing Key Laboratory of Health Cultivation of the Ministry of Education,Beijing University of Chinese Medicine,Beijing 100029,China)

机构地区：[1]北京中医药大学第三附属医院,北京100029 [2]中国人民解放军武装警察部队北京总队医院,北京100027 [3]北京中医药大学教育部中医养生学重点实验室,北京100029

出　　处：《世界中医药》2023年第19期2727-2731,共5页World Chinese Medicine

基　　金：国家自然科学基金青年科学基金项目(82004338);教育部优秀青年科学基金项目(2018-JYBZZ-JS169)。

摘　　要：目的:观察中药糖耐康对KKAy小鼠血糖、体质量及Toll样受体4(TLR4)通路中白细胞介素6(IL-6)、核因子κB激酶β抑制物(IKKβ)及葡萄糖转运蛋白4(GluT4)影响,分析其改善2型糖尿病的可能作用机制。方法:选取无特定病原体(SPF)级自发性2型糖尿病动物KKAy小鼠及C57BL/6J小鼠,给予高脂饲料喂养,连续8周给药,评价血糖及体质量。并采用半定量酶联免疫吸附测定(ELISA)检测血浆中IL-6及GluT4的水平。蛋白质印迹法检测肝组织TLR4蛋白表达、肌肉组织IKKβ蛋白表达水平。结果:中药糖耐康高剂量组,能够显著降低血糖水平(P<0.05),具有一定抑制体质量增长的趋势。同时能够下调IL-6水平,上调GluT4的水平(P<0.01,P<0.05),抑制TLR4及IKKβ蛋白表达(P<0.01,P<0.05)。结论:糖耐康可能通过下调脂多糖(LPS)介导的TLR4通路中关键炎症介质的水平,减轻低度炎症反应,在一定程度上抑制炎症级联效应,同时提高胰岛素信号转导受体后途径中GluT4的转位及活性水平,共同达到改善胰岛素抵抗状态的作用。Objective:To observe the effects of traditional Chinese medicine(TCM)compound Tangnai Kang on blood sugar,body weight,interleukin-6(IL-6)and inhibitor of nuclear factor kappaB kinase beta(IKKβ)in Toll-like receptor 4(TLR4)signaling pathway and glucose transporter 4(GluT-4)in KKAy mice,and to discuss its possible mechanism in improving type 2 diabetes mellitus(T2DM).Methods:SPF KKAy and C57BL/6J mice(spontaneous T2DM animals)were selected and fed with high fat food.After 8 weeks,blood sugar and body weight were evaluated and the levels of IL-6 and GluT-4 in plasma were measured by semi-quantitative ELISA.The protein expressions of TLR4 in liver tissue and IKKβin muscle tissue were detected by Western blot.Results:High dose of Tangnai Kang reduced blood glucose(P<0.05)and controlled the growth trend of body weight.It also decreased the level of IL-6 and increased the level of GluT-4(P<0.01,P<0.05),and inhibited the protein expressions of TLR4 in liver tissue and IKKβin muscle tissue(P<0.01,P<0.05).Conclusion:Tangnai Kang may reduce the low-grade inflammation response by down-regulating the key inflammatory factors in the LPS-mediated TLR4 signaling pathway,and thus inhibit the inflammatory cascade effect to some extent.It also enhances the transposition and activity of GluT-4 in the insulin signal transduction pathway,to jointly improve the insulin resistance.

关 键 词：糖耐康 自发性糖尿病小鼠 TLR4通路 白细胞介素6 核因子κB激酶β抑制物 葡萄糖转运蛋白4 作用机制 

分 类 号：R259[医药卫生—中西医结合]