酸枣仁-柏子仁组分调控睡眠障碍中神经递质的研究  

Study on the Regulation of Neurotransmitters in Sleep Disorders by Sour Jujube Kernels-Cypress Kernels Component

作　　者：倪雨婷 张欢欢 赵丹丹 赵玉艳 魏一璇 庞文俊 陈凤 郭洪麟 蒋希成 崔闻宇[1] 张乔[1] Ni Yuting(School of Pharmacy,Harbin University Commerce,Harbin 150076)

机构地区：[1]哈尔滨商业大学药学院,黑龙江哈尔滨150076 [2]黑龙江中医药大学基础医学院,黑龙江哈尔滨150040

出　　处：《黑龙江医药》2023年第5期1000-1004,共5页Heilongjiang Medicine journal

基　　金：国家级大学生创新创业训练计划项目(编号:202210240019X);国家自然科学基金(编号:82174261);黑龙江省中医药管理局中医药项目(编号:ZHY2022-046)。

摘　　要：目的:探讨宁心安神中药酸枣仁-柏子仁对小鼠下丘脑5-HT能神经通路表达的影响。方法:首先,实验通过检测酸枣仁-柏子仁有效组分对小鼠海马区、下丘脑部位以及前额叶皮质区色氨酸羟化酶(TPH)和5-HT含量变化,考察酸枣仁-柏子仁有效组分对小鼠海马区、下丘脑部位以及前额叶皮质区TPH和5-HT生成的影响;其次通过TRIZOL法提取小鼠下丘脑RNA进行逆转录,最后运用Real-time PCR技术检测5-HT转运体的编码基因Slc6a4基因及5-HT1A受体编码基因Htr1a表达比值,以此探讨酸枣仁-柏子仁有效组分对小鼠下丘脑5-HT转运体编码基因及5-HT1A受体编码基因表达的影响。结果:实验结果显示,与空白组相比,给药组小鼠下丘脑部位、海马区以及前额叶皮质区TPH和5-HT的含量均显著升高(P<0.05);酸枣仁-柏子仁有效组分能使小鼠下丘脑部位Slc6a4基因表达明显降低,而Htr1a基因表达明显增加。结论:实验结果表明酸枣仁-柏子仁有效组分能通过影响色氨酸羟化酶(TPH)、5-HT转运体编码基因Slc6a4基因及5-HT1A受体编码基因Htr1a基因来增强5-HT能神经系统的效应,进而来发挥改善睡眠的作用。Objective:To investigate the effect of effective components of Sour jujube kernels-cypress kernels Powder on the expression of 5-HT nerve pathway in hypothalamus of mice.Methods:The content of tryptophan hydroxylase(TPH)and 5-HT in different brain regions of mice was detected,and the effect of effective components of Sour jujube kernels-cypress kernels Powder on the production of 5-HT in different brain regions of mice was investigated.After retroviral transcription of mouse hypothalamic RNA extracted by TRIZOL method,the expression ratio of Slc6a4 gene encoding 5-HT transporter and Htr1a gene encoding 5-HT1A receptor was detected by Real-time PCR.The effect of active ingredients of Powder on the expression of 5-HT transporter coding gene and 5-HT1A receptor coding gene in hypothalamus of mice.Result:Compared with the blank group,the TPH and 5-HT concentration in hippocampus,hypothalamus and prefrontal cortex of mice in our jujube kernels-cypress kernels Powder group was significantly increased(P<0.05);the Slc6a4 gene expression in hypothalamus of mice was significantly decreased,while the Htr1a gene expression was significantly increased in our jujube kernels-cypress kernels Powder group.Conclusion:The experimental results show that the effective components of our jujube kernels-cypress kernels Powder can enhance the effect of 5-HT nervous system by affecting tryptophan hydroxylase(TPH),5-HT transporter coding gene Slc6a4 and 5-HT1A receptor coding gene Htr1a,thereby improving sleep.

关 键 词：酸枣仁-柏子仁 5-HT TPH Slc6a4 5-HT1A 

分 类 号：R285[医药卫生—中药学]