基于网络药理学对鹿茸抗骨质疏松症的作用机制研究  

Study on Action Mechanismof Pilose Antler Against Osteoporosis Based on the Network Pharmacology

作　　者：李春楠 申嘉明 吕经纬 张楠茜 张辉 孙佳明 LI Chunnan;SHEN Jiaming;LV Jingwei;ZHANG Nanxi;ZHANG Hui;SUN Jiaming(Jilin Ginseng Academy in Changchun University of Chinese Medicine,Changchun 130117,China)

机构地区：[1]长春中医药大学吉林省人参科学研究院,吉林长春130117

出　　处：《特产研究》2023年第6期87-97,共11页Special Wild Economic Animal and Plant Research

基　　金：吉林省卫生与健康科技能力提升计划项目(2020Q026);吉林省科技厅科技发展计划重点项目(20200404081YY)。

摘　　要：本研究基于网络药理学结合体外细胞试验,探究鹿茸抗骨质疏松症(Osteoporosis,OP)的作用机制。通过中药系统药理学成分分析平台(BATMAN-TCM)、中国知网和Web of Science等文献检索数据库对鹿茸主要成分进行收集,随后利用Swiss Target Prediction、SEA、Drugbank和Therapeutic Target Database(TTD)等数据库获得成分预测靶点和OP疾病靶点,交集后确定鹿茸抗OP关键核心靶点;应用Cytoscape 3.7.1软件中Clue GO插件和Metascape数据库对核心靶点进行GO和KEGG通路分析,并构建多层次网络图,分析拓扑参数;利用AutoDock软件将主要的活性成分与核心靶点进行分子对接验证,同时应用成骨细胞(MC3T3-E1)进一步验证鹿茸抗OP作用和网络药理学所得结果。网络药理学方法结合文献检索,最终确定鹿茸成分58个,预测成分靶点1331个,OP疾病靶点106个,筛选出SRC、IL6、TNF和PTGS2等鹿茸抗OP核心靶点33个;其主要参与流体剪切应力的细胞反应和药物跨膜转运等4个生物学过程;调控类固醇激素生物合成和雌激素信号等7类41条KEGG通路发挥作用;细胞试验验证了鹿茸具有很好的抗OP活性;分子对接和q-PCR验证了鹿茸抗OP作用机制。本研究明确了鹿茸多成分及多靶点的作用特点,为下一步其抗OP研究与产品开发提供理论依据。The study to explore the molecular mechanism of pilose antler against Osteoporosis(OP)based on network pharmacology and in vitro cell experiments.The main components of pilose antler were collected by logging into BATMAN-TCM and literature retrieval databases such as CNKI and Web of Science,and then the component prediction targets and OP disease targets were obtained from databases such as Swiss Target Prediction,SEA,Drugbank,Therapeutic Target Database(TTD),and the key core anti-OP targets of pilose antler were determined after intersection.The Clue GO plug-in in Cytoscape 3.7.1 software and the Metascape database were used to analyze the GO and KEGG pathways of the core targets,and a multi-level network diagram was constructed to analyze the topology parameters.The molecular docking verification of the main active components and core targets was performed using the AutoDock software,and the osteoblast(MC3T3-E1)was used to further verify the anti-OP effect of pilose antler and the network pharmacology conclusions.Based on the network pharmacology and literature retrieval,58 components of pilose antler were identified,including 1331 predicted component targets,106 OP disease targets,and 33 anti-OP core targets of pilose antler,including SRC,IL6,TNF and PTGS2,were screened out.It is mainly involved in four biological processes such as cell reaction under fluid shear stress and drug transmembrane transport.It regulated and controlled steroid hormone biosynthesis,estrogen signals and other 7 types of 41 KEGG pathways to play a role.Cell experiments verified that pilose antler had good anti-OPactivity.Molecular docking and q-PCR verified the anti-OP mechanism of pilose antler.This study explains the multi-component and multitarget action characteristics of pilose antler,and provides theoretical the basis for the next step of its anti-OP research and product development.

关 键 词：鹿茸 骨质疏松 成骨细胞 网络药理 

分 类 号：R285[医药卫生—中药学]