2020-2021年云南蔬菜产区番茄斑驳花叶病毒的发生及其豌豆分离物全基因组序列分析  

Occurrence of tomato mottle mosaic virus in Yunnan vegetable production ereas during 2020-2021 and complete genome amplification and sequence analysis of a ToMMV pea isolate

作　　者：张舒 张天毅 陈泓霖 谭冠林 李凡[1] ZHANG Shu;ZHANG Tianyi;CHEN Honglin;TAN Guanlin;LI Fan(College of Plant Protection,Yunnan Agricultural University,Kunming 650201,China;Modern Education Technology Center,Yunnan Agricultural University,Kunming 650201,China)

机构地区：[1]云南农业大学植物保护学院,昆明650201 [2]云南农业大学现代教育技术中心,昆明650201

出　　处：《植物病理学报》2023年第5期819-830,共12页Acta Phytopathologica Sinica

基　　金：国家自然科学基金(31660039);云南省院士专家工作站(202005AF150040)。

摘　　要：云南是国内首个发现番茄斑驳花叶病毒(tomato mottle mosaic virus, ToMMV)的省份之一。自2013年在元谋县的辣椒上发现ToMMV以来,截至目前已在云南省多个地区的辣椒和番茄上发现了ToMMV的侵染。为明确2020-2021年间ToMMV在云南省的发生分布情况,对云南省7个州市蔬菜产区的田间植物样品进行了ToMMV的分子检测。发现ToMMV仅在楚雄州元谋县的番茄、红河州蒙自市和昆明市晋宁区的豌豆上零星发生。其中,2020年ToMMV的检出率为1.79%,2021年为1.41%。豌豆是继茄科作物外新发现的ToMMV自然新寄主,染病豌豆顶端叶片黄化,伴有轻微花叶和皱缩。利用RT-PCR及RACE技术获得了ToMMV豌豆分离物(GenBank登录号:ON146334)全基因组序列6 400 nt,其与国内外已报道的ToMMV分离物核苷酸序列一致性在98.53%~99.95%之间,这是首例从非茄科植物上分离出来的ToMMV全基因组序列,研究结果有助于进一步了解该病毒种群的遗传进化关系。本文的结果也表明,无论是来自茄科寄主还是非茄科寄主,当前国内外发现的ToMMV的遗传变异不大,可能有共同的起源。Yunnan is one of the first provinces in China to record the occurrence of tomato mottle mosaic virus(ToMMV).Since ToMMV was detected on pepper in Yuanmou County in 2013,ToMMV infection has been found in pepper and tomato plants in many areas of Yunnan Province so far.In order to clarify the occurrence and distribution of ToMMV in Yunnan Province during 2020 and 2021,molecular detection of ToMMV was carried out on field plant samples from vegetable producing areas in seven cities/autonomous prefectures of Yunnan Province.The virus detection results indicated that ToMMV only occurred sporadically on tomato in Yuanmou County of Chuxiong Prefecture,and on pea in Mengzi City of Honghe Prefecture and Jinning District of Kunming City.Among them,the detection rate of ToMMV was 1.79%in 2020 and 1.41%in 2021.Pea(family Leguminosae)is one of the new natural hosts of ToMMV besides solanaceous crops,and the apical leaves of the affected pea plant showed yellowing as well as slight mosaic and shrinking.The 6400 nt full-length genome sequence of ToMMV pea isolate(ToMMV-MZWD,GenBank accession number:ON146334)was obtained by RT-PCR and RACE.Sequence comparison analysis results revealed that the genome sequence of ToMMV-MZWD shared 98.53%to 99.95%nucleotide sequence identity with other ToMMV isolates around the world.This is the first complete genome sequence of ToMMV isolated from a non-solanaceous crop,and it will help to further understand the genetic evolution of the viral population.The results of this paper also manifested that the genetic variation of ToMMV currently found in China and other countries,whether from Solanaceae or non-solanaceae hosts,is not significant,and suggested a common origin.

关 键 词：番茄斑驳花叶病毒 发生 豌豆 基因组序列 

分 类 号：S432.41[农业科学—植物病理学]