引起莴苣灰霉病的灰葡萄孢红色菌系的分离及鉴定  

Isolation and identification of a red strain of Botrytis cinerea causing gray mold on lettuce

作　　者：刘帆兴 宋莉萍 余楚英 林处发 汤利光 汪爱华 LIU Fanxing;SONG Liping;YU Chuying;LIN Chufa;TANG Liguang;WANG Aihua(Wuhan Academy of Agricultural Sciences,Wuhan 430065,China;Huazhong Agricultural University,Wuhan 430070,China)

机构地区：[1]武汉市农业科学院,武汉430065 [2]华中农业大学,武汉430070

出　　处：《植物病理学报》2023年第5期966-969,共4页Acta Phytopathologica Sinica

基　　金：财政部和农业农村部:国家现代农业产业技术体系资助(CARS-23-G27);湖北省博士后资助项目;武汉市农业科学院创新体系建设青年创新项目(QNCX202102)。

摘　　要：灰葡萄孢(Botrytis cinerea)是一种死体营养型植物病原真菌,寄主范围广泛,可以侵染1 400多种植物,其中包括葡萄、番茄、草莓、莴苣等具重要经济价值的作物[1]。前人根据灰葡萄孢分生孢子的产生和分布以及菌核的有无,将形态表型分为菌丝型、孢子型和菌核型[2]。科研人员还根据灰葡萄孢菌核的颜色将其分类为黑色菌系、黄色菌系和黄黑色菌系(菌核开始为黄色,最终变为黑色)[3]。2021年4月,课题组在湖北武汉调查莴苣病害发生情况时观察到一株疑似能够产生红色分泌物的病原菌。经单孢分离、形态学观察、致病力测定以及分子生物学鉴定,明确了其分类学地位。本研究旨在拓展人们对于灰葡萄孢遗传多样性的认识,并为灰霉病的综合防治提供参考。Gray mold,caused by the necrotrophic fungal pathogen Botrytis cinerea,is one of the most devasta-ting diseases on lettuce.In this study,tissue isolation was performed to obtain a pure culture that caused necrotic symptoms on lettuce.In order to identify the pathogen,single spore isolation,morphological observation,pathogenicity determination,sequence-characterized amplified region(SCAR)marker detection,and phyloge-netic analysis were conducted.The results showed that this pathogen appeared red when grown on potato dextrose agar(PDA)plates and was identified as B.cinerea.This is the first report of a red strain of B.cinerea.

关 键 词：灰葡萄孢 病害发生情况 寄主范围 菌系 单孢分离 植物病原真菌 灰霉病 科研人员 

分 类 号：S432.44[农业科学—植物病理学]