北京市猴痘病例不同类型临床标本的检测分析  

Detection of mpox cases in Beijing using different types clinical specimen

作　　者：孙玉兰[1] 金昊源 李夫 窦相峰[1] 任振勇[1] 李丹 李仁清[1] 李伟红[1] 张新[1] 杨扬[1] 张代涛[1] Sun Yulan;Jin Haoyuan;Li Fu;Dou Xiangfeng;Ren Zhenyong;Li Dan;Li Renqing;Li Weihong;Zhang Xin;Yang Yang;Zhang Daitao(Institute for Infectious Disease and Endemic Disease Control,Beijing Center for Diseases Prevention and Control,Beijing 100013,China)

机构地区：[1]北京市疾病预防控制中心传染病地方病控制所,100013

出　　处：《国际病毒学杂志》2023年第5期361-364,共4页International Journal of Virology

摘　　要：目的探索猴痘病毒在不同类型临床标本中的检出规律,为猴痘病例的诊断及公共卫生政策的制定提供依据。方法采集病例的咽拭子、皮损拭子、血液和肛拭子标本,使用实时荧光定量PCR方法检测标本中的猴痘病毒DNA。分析不同类型标本阳性检出率的差异,并使用Prism软件分析不同类型标本病毒载量(Ct值)差异,以及同一类型标本在不同的采样间隔组之间其病毒载量(Ct值)的差异。结果210例确诊病例的咽拭子、皮损拭子、血液、和肛拭子标本的阳性检出率分别为70.95%、98.97%、75.26%和88.89%。咽拭子组、皮损拭子组、血液组和肛拭子组的Ct值中位数分别为31、17、34和26,对于皮损拭子和肛拭子两类标本,根据病例采样间隔的长短不同,病毒载量(Ct值中位数)具有统计学差异。结论皮损拭子是检测猴痘病毒的最佳标本类型,其他类型标本可作为替代或补充。Objective To explore the the detection patterns of mpox virus in different types of clinical specimen,so as to provide evidence for the diagnosis of mpox cases and the formulation of public health policies.Methods The throat swabs,skin lesion swabs,blood and anal swabs from the cases were collected.Real-time quantitative PCR method was used to detect monkeypox virus DNA in the samples.The differences in the positive detection rate of different sample types were analyzed the Prism software was used to analyze the differences in the viral loads(Ct values)in different types of samples and in different sampling time of the same sample type.Results The positive detection rates of throat swabs,skin lesion swabs,blood,and anal swabs in the 210 confirmed cases were 70.95%,98.97%,75.26%and 88.89%,respectively.The median Ct values for the throat swab group,skin lesion swab group,blood group,and anal swab group were 31,17,34,and 26,respectively.The difference in viral loads(Ct values)among patient groups with different sampling intervals for skin lesion swab group and anal swab group were statistically significant.Conclusions Skin lesions should be the optimal specimen type for detecting mpox virus,while other types of specimens can be used as alternatives or supplements.

关 键 词：猴痘病毒 病毒载量 临床标本 皮损拭子 

分 类 号：R511[医药卫生—内科学]