急性胰腺炎小鼠胰腺和远隔脏器组织TRAF6、XIAP表达变化及其与细胞凋亡的关系  

Expression changes of TRAF6 and XIAP in pancreas and distal organs of mice with acute pancreatitis and their relationship with apoptosis

作　　者：谭贞菊 周翔宇 陈霞 TAN Zhenju;ZHOU Xiangyu;CHEN Xia(Department of Gastroenterology,The Affiliated Hospital of Southwest Medical University,Luzhou 646000,China;不详)

机构地区：[1]西南医科大学附属医院消化内科,四川泸州646000 [2]西南医科大学附属医院甲状腺血管外科 [3]成都医学院第一附属医院消化内科

出　　处：《山东医药》2023年第32期45-49,共5页Shandong Medical Journal

基　　金：四川省医学科研课题计划项目(S22039)。

摘　　要：目的观察急性胰腺炎小鼠胰腺和远隔脏器组织中肿瘤坏死因子受体相关因子6(TRAF6)和X连锁凋亡抑制蛋白(XIAP)表达变化,并分析其与多脏器细胞凋亡的关系。方法将30只雄性C57BL/10SnJ小鼠随机分为对照组、急性水肿性胰腺炎(AEP)组、急性坏死性胰腺炎(ANP)组,每组10只。AEP组建立AEP模型,ANP组建立ANP模型,对照组注射等量磷酸盐缓冲盐水。采用RT-qPCR法检测各组胰腺组织中的TRAF6、XIAP mRNA,Western blotting法检各组胰腺、肠、肝、肺、肾组织中的cleaved Caspase-3、TRAF6、XIAP蛋白。采用TUNEL法测算胰腺、小肠、肝、肺、肾组织细胞凋亡率。结果ANP组、AEP组胰腺及多个远隔脏器组织中TRAF6、XIAP蛋白表达与对照组比较差异均有统计学意义(P均<0.05);AEP组胰腺组织中XIAP mRNA表达低于ANP组(P<0.05);AEP组胰腺、肠、肝、肺、肾组织中TRAF6蛋白表达高于ANP组,XIAP蛋白表达低于ANP组(P均<0.05)。AEP组胰腺、肠、肝、肺、肾组织细胞凋亡率及cleaved Caspase-3表达高于ANP组和对照组(P均<0.05)。胰腺炎小鼠胰腺及多个远隔脏器组织中cleaved Caspase-3蛋白表达与TRAF6蛋白表达呈正相关,与XIAP蛋白表达呈负相关(P均<0.05)。结论急性胰腺炎小鼠胰腺和远隔脏器组织中TRAF6、XIAP表达异常,且与细胞凋亡指标存在相关性;TRAF6、XIAP在AEP与ANP中的表达趋势有所差异,TRAF6、XIAP对细胞凋亡的调控作用可能与急性胰腺炎严重程度有关。Objective To observe the expression changes of tumor necrosis factor receptor-associated factor 6(TRAF6)and X-linked apoptosis inhibitory protein(XIAP)in the pancreas and distal organs of mice with acute pancreatitis and to analyze their relationship with apoptosis in multiple organs.Methods Thirty male C57BL/10SnJ mice were randomly divided into the control,acute edematous pancreatitis(AEP),and acute necrotizing pancreatitis(ANP)groups,with 10 mice in each group.In the AEP group,we established the AEP models;in the ANP group,we established the ANP models;in the control group,mice were injected with an equal volume of phosphate-buffered saline.TRAF6 and XIAP mRNA in pancreas,intestine,liver,lung and kidney tissues of each group were detected by RT-qPCR,and cleaved Caspase-3,TRAF6 and XIAP proteins were detected by Western blotting.The apoptosis rates of pancreas,small intestine,liver,lung and kidney tissues were measured by TUNEL method.Results Statistically significant differences were found in the TRAF6 and XIAP protein expression in the pancreas and several distal organs between the ANP and AEP groups and the control group(all P<0.05),XIAP mRNA expression in the pancreatic tissues of the AEP group was lower than that in the ANP group(P<0.05),TRAF6 protein expression in the pancreatic,intestinal,hepatic,lung,and renal tissues of the AEP group was higher than that of the ANP group,and XIAP protein expression was lower than that in the ANP group(all P<0.05).The apoptosis rate and cleaved Caspase-3 expression in the pancreatic,intestinal,liver,lung and kidney tissues in the AEP group were higher than those in the ANP group and the control group(all P<0.05).The expression of cleaved Caspase-3 protein in the pancreas and several distal organs of pancreatitis mice was positively correlated with TRAF6 protein expression and was negatively correlated with XIAP protein expression(all P<0.05).Conclusions The expression of TRAF6 and XIAP in the pancreas and distal organ tissues of mice with acute pancreatitis is abnormal

关 键 词：急性水肿性胰腺炎 急性坏死性胰腺炎 急性胰腺炎 细胞凋亡 肿瘤坏死因子受体相关因子6 X连锁凋亡抑制蛋白 

分 类 号：R657.5[医药卫生—外科学]