2011-2020年福建省手足口病相关柯萨奇病毒A9型基因特征及重组分析  

Genetic characteristics and recombination analysis of HFMD related Coxsackievirus A9 in Fujian province from 2011 to 2020

作　　者：何文祥[1] 李林丰 朱颖[1] 翁育伟[1,2] 陈炜[1,2] HE Wenxiang;LI Linfeng;ZHU Ying;WENG Yuwei;CHEN Wei(Fujian Provincial Center for Disease Control and Prevention Fujian Provincial Key Laboratory of Zoonosis Research,Fuzhou 350001,China;School of Public Health,Fujian Medical University)

机构地区：[1]福建省疾病预防控制中心,福建省人兽共患病研究重点实验室,福建福州350001 [2]福建医科大学公共卫生学院

出　　处：《中国病原生物学杂志》2023年第12期1370-1374,共5页Journal of Pathogen Biology

基　　金：国家科技重大专项课题(No.2017ZX10104001);福建省卫生健康科技计划项目(No.2021GGB012)。

摘　　要：目的了解福建地区手足口病(hand,foot and mouth disease,HFMD)相关柯萨奇病毒A9型(coxsackievirus A9,CV-A9)的基因特征及重组情况。方法对2011-2020年福建省各地市上送的HFMD阳性标本进行CV-A9筛查、VP1区全长序列测定和基因型别鉴定及分析。对CV-A9阳性标本利用人横纹肌肉瘤细胞(rhabdomyosarcoma,RD)及人喉癌上皮细胞(Human laryngeal carcinoma epithelial cells,HEp-2)进行病毒分离,对分离毒株进行全基因组测序及重组分析。结果2011-2020年福建省各地市上送的HFMD阳性标本中共鉴定出HFMD相关CV-A9阳性7份,获得6条VP1区全长序列。基于VP1序列将CV-A9划分为A~O 15个基因型,其中G基因型又可分为G1及G2两个基因亚型。福建省CV-A9均属于G2基因亚型,在系统进化树上呈散在分布。分离并测序获得3条CV-A9全基因组序列,分析显示毒株2014FJFZ463与福建地区流行的ECHO30毒株存在重组。结论G2基因亚型是我国现阶段流行的优势型别。2011-2020年福建省HFMD相关CV-A9流行呈散发状态,未发现本土传播链的形成,但存在基因重组株。Objective To investigate the genetic characteristics and recombination of hand,foot,and mouth disease(HFMD)related coxsackievirus A9(CV-A9)in Fujian province.Methods Screening CV-A9from positive HFMD samples which collected from all cities in Fujian province from 2011 to 2020,then sequencing VP1 full-length region to identify genotypes and analyzing.Human rhabdomyosarcoma(RD)and Human laryngeal carcinoma epithelial cells(HEp-2)were used for virus isolation in CV-A9 positive samples,the whole genome of isolated strains was sequenced to analyze viral gene recombination.Results A total of 7 HFMD-associated CV-A9 positive samples were identified in Fujian province from 2011 to 2020,and obtained 6 genetic sequences of CV-A9 VP1.Base on the VP1 sequence,CV-A9 was classified into A-O genotypes,the average nucleotide difference among 15 genotypes was 15.2%-26.1%.G genotype was further classified G1 and G2 subgenotype,the average nucleotide difference between two sbugenotypes was 13.7%.All CV-A9 in Fujian province belongs to G2 subgenotype which wasdistributed scattered in the phylogenetic tree.Three complete genome sequences of CV-A9 were isolated and sequenced.Compared with the CV-A9 prototype Griggs,the homology in P1 region of three sequences was higher(81.0%-81.5%)than that in P2 and P3 region,and was closest to the homology of complete sequence(80.1%-81.2%).The Simplot analysis results showed there was recombination between CV-A9 strain 2014FJFZ463 and ECHO30,strains prevalent in Fujian region.Conclusion G2 subgeotype is the dominant type prevalent in China.The HFMD-associated CV-A9 epidemic in Fujian province from 2011 to 2020 was sporadic.Local transmission chain was not found,but there were gene recombination strain existed.

关 键 词：柯萨奇病毒A9型 基因分析 重组 

分 类 号：R373.23[医药卫生—病原生物学]