干旱胁迫下枯草芽孢杆菌对玉米苗期叶片的生理生化调节  

Physiological and Biochemical Regulation of Bacillus subtilis Spores on Leaves at Seedling Stage of Maize under Drought Stress

作　　者：李安 舒健虹[2] 刘晓霞 路雪萍 焦渔 王小利[2] 赵德刚 LI An;SHU Jianhong;LIU Xiaoxia;LU Xueping;JIAO Yu;WANG Xiaoli;ZHAO Degang(Guizhou Maotai Wine Factory Group Health Care Wine Industry Co.,Ltd,Zunyi Guizhou 563000,China;Institute of Grass Industry,Guizhou Academy of Agricultural Sciences,Guiyang 550006,China;Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region,Ministry of Education,Collaborative Innovation Center for Mountain Ecology&Agro-Bioengineering(CICMEAB),College of Life Sciences/Institute of Agro-bioengineering,Guizhou University,Guiyang 550025,China;College of Brewing and Food Engineering,Guizhou University,Guiyang 550020,China)

机构地区：[1]贵州茅台酒厂(集团)保健酒业有限公司,贵州遵义563000 [2]贵州省农业科学院草业研究所,贵阳550006 [3]贵州大学生命科学学院/农业生物工程研究院,山地植物资源保护与种质创新教育部重点实验室,山地生态与农业生物工程协同创新中心,贵阳550025 [4]贵州大学酿酒与食品工程学院,贵阳550020

出　　处：《西北农业学报》2023年第12期1964-1977,共14页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：贵州省高层次创新人才培养项目[黔科合平台人才(2018)5634-2];贵州省科技计划项目[黔科合平台人才(2020)5005];贵州省人才培养项目;贵州省高层次创新型人才培养项目[黔科合人才(2016)4003]。

摘　　要：为探究干旱胁迫下不同枯草芽孢杆菌对玉米苗期叶片的生理调节作用,通过对干旱与正常水分下玉米根部接种R9-1、R59、R60等3种不同植物根际的枯草芽孢杆菌及R59+R60组合菌种,测定玉米苗期叶片的抗氧化酶活性、细胞渗透物质、细胞膜氧化产物、H_(2)O_(2)、内源激素、相对含水量等生理生化调节物质以及叶片的K、P、N含量。通过主成分分析与相关性分析发现接种枯草芽孢杆菌主要影响玉米苗期叶片的可溶性糖、脱落酸(ABA)、磷(P)含量、超氧化歧化酶(SOD)活性、过氧化氢酶(CAT)活性、过氧化物酶(POD)活性、叶片游离脯氨酸(PRO)含量、叶片相对含水量,其中叶片相对含水量与其他生理表现因子呈显著的负相关关系。干旱胁迫下,接种枯草芽孢杆菌的玉米苗期叶片抗氧化酶活性、细胞渗透调节物质、P、N含量显著降低。接种R9-1、R59降低H2O2与ABA含量,接种R60、R59+R60提高ABA含量,接种R59、R60、R59+R60降低吲哚乙酸含量。隶属函数综合分析枯草芽孢杆菌对玉米的调节作用排名为:正常情况下R60>R59>R9-1>R59+R60>CK1;干旱胁迫下R60>R59+R60>R59>R9-1>CK2。综上所述,接种枯草芽孢杆菌玉米苗期叶片的生理表现为抗氧化酶活性、细胞渗透调节物质、P、N含量显著降低,同时R59、R60、R59+R60均有效提升玉米苗期叶片的相对含水量,说明接种枯草芽孢杆菌玉米苗期受干旱胁迫影响程度较小,所以接种枯草芽孢杆菌具有提升玉米苗期适应干旱胁迫的调节能力,从而保证自身的生长发育。In order to explore the physiological regulation effects of different Bacillus subtilis on the leaves of maize seedlings under drought stress.The physiological and biochemical regulating substances,such as the antioxidant enzyme activity,cell permeation substances,cell membrane oxidation products,H_(2)O_(2),endogenous hormones,relative water content,and K,P,N contents in the leaves of maize seedlings were evaluated by inoculating maize roots with different plant rhizosphere Bacillus subtilis,which include R9-1,R59,R60 and R59+R60 combination strains under drought and normal water conditions.The principal component and correlation analysis indicated that Bacillus subtilis mainly affected the soluble sugar,abscisic acid(ABA),phosphorus(P)content,superoxide dismutase(SOD)activity,catalase(CAT)activity,peroxidase(POD)activity,free proline(PRO)content,relative water content of the leaves at seedling stageof maize.Importantly,there was a significantly negative correlation between the relative water content and other physiological performance factors.Under drought stress,the antioxidant enzyme activity,cell osmotic adjustment substances,and P and N content in the leaves of maize seedlings obviously decreased after inoculated with Bacillus subtilis.By the way,the content of H_(2)O_(2) and ABA reduced after R9-1,R59 inoculation,the content of ABA increased after R60,R59+R60 inoculation and the content of IAA decreased after R59,R60,and R59+R60 inoculation in the leaves of maize at seedling stage.The regulation effect of Bacillus subtilis on maize was estimated by the comprehensive analysis of membership function under different conditions.Under normal conditions,R60>R59>R9-1>R59+R60>CK1,under drought stress,R60>R59+R60>R59>R9-1>CK2.Taken together,the antioxidant enzyme activity,cell osmotic regulation substances,and P,N content of leaves at seedling of maize decreased after inoculation with Bacillus subtilis.Meanwhile,R59,R60,R59+R60 was conducive to improve its relative water content.The results demonstrated that the mai

关 键 词：玉米 枯草芽孢杆菌 隶属函数 干旱胁迫 

分 类 号：S513[农业科学—作物学]