沙棘rbcL基因的扩增及序列分析  

Amplification and Sequence Analysis of rbcLGene in Hippophae rhamnoides

作　　者：刘青青 杨开宇 马玉花[1] 施会 冶贵生[1] 冯智鹏[1] LIU Qingqing;YANG Kaiyu;MA Yuhua;SHI Hui;YE Guisheng;FENG Zhipeng(College of Agriculture and Animal Husbandry,Qinghai University,Xining,Qinghai 810016;Academy of Animal Husbandry and Veterinary Sciences,Qinghai University,Xining,Qinghai 810016;Chengdu Nature Reserve and Wildlife Conservation Center,Chengdu,Sichuan 610031)

机构地区：[1]青海大学农牧学院,青海西宁810016 [2]青海大学畜牧兽医科学院,青海西宁810016 [3]成都市自然保护地和野生动植物保护中心,四川成都610031

出　　处：《北方园艺》2023年第21期42-52,共11页Northern Horticulture

基　　金：国家自然科学基金资助项目(31660071);青海省科技厅资助项目(2017-ZJ-734)。

摘　　要：以青海省11个地区的中国沙棘以及祁连县的西藏沙棘和肋果沙棘为试材,采用叶绿体基因标记rbcL构建遗传距离矩阵及系统发育树,研究了青海省不同地区中国沙棘的遗传变异,以期为沙棘植物分类研究提供参考依据。结果表明:3种沙棘属植物的rbcL区长度均为1501bp,不存在长度变异;3种沙棘属植物的rbcL基因序列存在不同的变异位点,不同地区中国沙棘的rbcL基因间也存在少量变异位点;与NCBI上的部分rbcL基因序列共同聚类分析发现形态手段与分子手段对沙棘进行系统分类具有基本一致性。该研究为未来利用rbcL序列进行沙棘系统进化研究提供了新的思路与方法,为沙棘的开发与利用奠定了基础。Taking Hippophae rhamnoides from 11regions in Qinghai Province and Tibetan sea buckthorn and ribbed sea buckthorn from Qilian county as test materials,agenetic distance matrix and phylogenetic tree were constructed using chloroplast gene marker rbcLto study the genetic variation of Hippophae rhamnoides in different regions of Qinghai Province,in order to provide reference for the classification of sea buckthorn plants.The results showed that the length of the rbcLregion of all three species of sea buckthorn plants was 1501bp,and there was no length variation.There were different variation sites in the rbcLgene sequences of three species of sea buckthorn plants,and there were also a small number of variation sites between the rbcL genes of Hippophae rhamnoides in different regions.Cluster analysis with some rbcLgene sequences on NCBI revealed basic consistency between morphological and molecular methods for systematic classification of sea buckthorn.This study provided new ideas and methods for future research on the phylogenetic evolution of sea buckthorn using rbcL sequences,laying the foundation for the development and utilization of sea buckthorn.

关 键 词：中国沙棘 RBCL 序列分析 变异 系统分类 

分 类 号：S793.6[农业科学—林木遗传育种]