Whole‑genome transcriptome and DNA methylation dynamics of pre‑implantation embryos reveal progression of embryonic genome activation in buffaloes  

作　　者：Penghui Fu Du Zhang Chunyan Yang Xiang Yuan Xier Luo Haiying Zheng Yanfei Deng Qingyou Liu Kuiqing Cui Fei Gao Deshun Shi 

机构地区：[1]State Key Laboratory for Conservation and Utilization of Subtropical Agro‑Bioresources&Guangxi Key Laboratory of Animal Breeding and Disease Control,Guangxi University,Nanning 530004,China. [2]College of Animal Science and Technology,Southwest University,Chongqing 402460,China. [3]Genome Analysis Laboratory of the Ministry of Agriculture,Agricultural Genomics Institute at Shenzhen,Chinese Academy of Agricultural Sciences,Shenzhen 518120,China. [4]Guangxi Key Laboratory of Buffalo Genetics,Reproduction and Breeding,Guangxi Buffalo Research Institute,Chinese Academy of Agricultural Science,Nanning 530001,China [5]Guangxi Academy of Medical Sciences and the People’s Hospital of Guangxi Zhuang Autonomous Region,Nanning 530016,China. [6]Guangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding School of Life Science and Engineering,Foshan University,Foshan 528225,China. [7]Comparative Pediatrics and Nutrition,Department of Veterinary and Animal Sciences,Faculty of Health and Medical Sciences,University of Copenhagen,DK 1870 C Frederiksberg,Denmark.

出　　处：《Journal of Animal Science and Biotechnology》2023年第6期2411-2427,共17页畜牧与生物技术杂志（英文版）

基　　金：funded by the National Natural Science Foundation of China (31972996 and 32160790);Guangxi Bagui Scholar Program;Guangxi Innovation-Driven Development Project (AA17204051)

摘　　要：Background During mammalian pre-implantation embryonic development(PED),the process of maternal-to-zygote transition(MZT)is well orchestrated by epigenetic modification and gene sequential expression,and it is related to the embryonic genome activation(EGA).During MZT,the embryos are sensitive to the environment and easy to arrest at this stage in vitro.However,the timing and regulation mechanism of EGA in buffaloes remain obscure.Results Buffalo pre-implantation embryos were subjected to trace cell based RNA-seq and whole-genome bisulfite sequencing(WGBS)to draw landscapes of transcription and DNA-methylation.Four typical developmental steps were classified during buffalo PED.Buffalo major EGA was identified at the 16-cell stage by the comprehensive analy-sis of gene expression and DNA methylation dynamics.By weighted gene co-expression network analysis,stage-spe-cific modules were identified during buffalo maternal-to-zygotic transition,and key signaling pathways and biological process events were further revealed.Programmed and continuous activation of these pathways was necessary for success of buffalo EGA.In addition,the hub gene,CDK1,was identified to play a critical role in buffalo EGA.Conclusions Our study provides a landscape of transcription and DNA methylation in buffalo PED and reveals deeply the molecular mechanism of the buffalo EGA and genetic programming during buffalo MZT.It will lay a foundation for improving the in vitro development of buffalo embryos.

关 键 词：BUFFALO DNA methylome Embryonic genome activation Maternal-to-zygote transition TRANSCRIPTOME 

分 类 号：S823[农业科学—畜牧学]