Punch‑excised explants of bovine mammary gland to model early immune response to infection  

作　　者：Pablo Gomes Noleto Florence B.Gilbert Christelle Rossignol Patricia Cunha Pierre Germon Pascal Rainard Rodrigo Prado Martins 

机构地区：[1]ISP,INRAE,Universitéde Tours,UMR1282,Nouzilly,France

出　　处：《Journal of Animal Science and Biotechnology》2023年第6期2649-2662,共14页畜牧与生物技术杂志（英文版）

基　　金：FEDER/Region Centre Val de Loire ANIMALT grant (FEDER convention number EX007516, Region Centre convention number 2019–00134936, research program number AE-2019–1850);a post-doc fellowship from INRAE–Département Santé Animale

摘　　要：Background Mammary gland(MG)infections(mastitis)are frequent diseases of dairy cows that affect milk quality,animal welfare and farming profitability.These infections are commonly associated with the bacteria Escherichia coli and Staphylococcus aureus.Different in vitro models have been used to investigate the early response of the MG to bacteria,but the role of the teat in mastitis pathogenesis has received less attention.In this study,we used punch-excised teat tissue as an ex vivo model to study the immune mechanisms that arise early during infection when bacteria have entered the MG.Results Cytotoxicity and microscopic analyses showed that bovine teat sinus explants have their morphology and viability preserved after 24 h of culture and respond to ex vivo stimulation with TLR-agonists and bacteria.LPS and E.coli trigger stronger inflammatory response in teat when compared to LTA and S.aureus,leading to a higher produc-tion of IL-6 and IL-8,as well as to an up-regulation of proinflammatory genes.We also demonstrated that our ex vivo model can be applied to frozen-stored explants.Conclusions In compliance with the 3Rs principle(replacement,reduction and refinement)in animal experimenta-tion,ex vivo explant analyses proved to be a simple and affordable approach to study MG immune response to infec-tion.This model,which better reproduces organ complexity than epithelial cell cultures or tissue slices,lends itself particularly well to studying the early phases of the MG immune response to infection.

关 键 词：Bovine teat Escherichia coli EXPLANT MASTITIS Staphylococcus aureus 3Rs 

分 类 号：S858.23[农业科学—临床兽医学]