Evaluation of Holstein cows with different tongue‑rolling frequencies:stress immunity,rumen environment and general behavioural activity  

作　　者：Fuyu Sun Xiaoyang Chen Yongfeng Li Guangyong Zhao Xianhong Gu 

机构地区：[1]State Key Laboratory of Animal Nutrition,Institute of Animal Science,Chinese Academy of Agricultural Sciences,Haidian District,No.2 Yuanmingyuan West Road,Beijing,China. [2]College of Animal Science and Technology,China Agricultural University,Haidian District,No.2 Yuanmingyuan West Road,Beijing,China. [3]Agricultural Information Institute,Chinese Academy of Agriculture Sciences,Haidian District,No.12 Zhongguancun South Street,Beijing,China

出　　处：《Journal of Animal Science and Biotechnology》2023年第6期2686-2700,共15页畜牧与生物技术杂志（英文版）

基　　金：the National Natural Science Foundation of China(32272926);the Agricultural Science and Technology Innovation Program(ASTIP-IAS07,Beijing,China).

摘　　要：Background The tongue-rolling behaviour of cows is regarded as an outward sign of stressed animals in a low welfare status.The primary aim of this observational study was to evaluate the association between the frequency of tongue-rolling behaviour and its physiological function.The secondary aim was to explore the relationship between general activities and the frequency of tongue-rolling behaviour of cows.A total of 126 scan sampling behavioural observations were collected over 7 d on 348 Holstein cows with the same lactation stage in the same barn.The tongue-rolling frequency was defined as the number of tongue-rolling observations as a percentage to the total observations per individual cow.According to their tongue-rolling frequency,the cows were grouped into the CON(no tongue-rolling),LT(frequency 1%),MT(frequency 5%),and HT(frequency 10%)groups.Six cows from each group were randomly selected for sampling.Serum samples,rumen fluid,milk yield,and background infor-mation were collected.The general behaviour data during 72 continuous hours of dairy cows,including eating time,rumination time,food time(eating time+rumination time),and lying time,were recorded by the collar sensor.Results Cortisol(P=0.012),γ-hydroxybutyric acid(P=0.008),epinephrine(P=0.030),and dopamine(P=0.047)levels were significantly higher in tongue-rolling groups than in the CON group.Cortisol levels and tongue-rolling frequency had a moderate positive correlation(linearly r=0.363).With the increase in tongue-rolling frequency,the rumen pH decreased first and then increased(P=0.013),comparing to the CON group.HT cows had significantly less food time than CON cows(P=0.035).The frequency of tongue-rolling had a moderate negative relationship with rumination time(r=-0.384)and food time(r=-0.492).Conclusions The tongue-rolling behaviour is considered as a passive coping mechanism,as the stress response in cows with high tongue-rolling frequency increased.Food intake and rumination activities were all closely related to the occurrence of tongue-

关 键 词：General activity Holstein cow Rumen fermentation Stress immunity Tongue rolling 

分 类 号：S823[农业科学—畜牧学]