mRNAs/microRNAs调控网络探索石斛花型差异分子机理研究  

Differential Molecular Mechanism of Dendrobium Flower Pattern Using mRNAs/microRNAs Regulatory Network

作　　者：臧睿 陈宇 赵美 郝玉恒 敖叠 李凯莉 方诗迪 赵奇明 和凤美[1] ZANG Rui;CHEN Yu;ZHAO Mei;HAO Yuheng;AO Die;LI Kaili;FANG Shidi;ZHAO Qiming;HE Fengmei(College of Horticulture and Landscape,Yunnan Agricultural University,Kunming,Yunnan 650201,China)

机构地区：[1]云南农业大学园林园艺学院,云南昆明650201

出　　处：《热带作物学报》2023年第11期2179-2187,共9页Chinese Journal of Tropical Crops

基　　金：国家自然科学基金项目(No.32260419);研究生创新课题(No.2022ZKY463)。

摘　　要：目前兰科植物花型多样性形成分子机理仍不清楚,本研究对铁皮石斛、美花石斛花器官进行转录组和microRNA测序,通过cytoscape构建miRNA-mRNA网络和靶基因蛋白互作网络并进行差异比较,以期在分子水平上探究2种石斛花型差异分子机理。转录组研究结果表明,与花发育相关的mRNAs有MADS-box AP3基因、激素类基因等。miRNA-mRNA网络分析表明,差异表达的miRNAs、mRNAs参与了花型差异形成,miR5179与靶基因AP3、miR160与靶基因ARFs、miR164与靶基因NAC021、miR159与靶基因GAM1、bdi-miR159与靶基因CKX9、miR167b-3p与靶基因ANT可能参与花型形成,以上miRNA均在铁皮石斛中上调表达。蛋白互作通路表明,2种石斛有AP3这一关键基因,美花石斛缺少CUL1、TIR1、TIR1A、RBS四个基因;2种石斛的生长素反应互作通路中存在2个差异表达的ARFs基因,美花石斛缺失1个ARFI基因;美花石斛特异性地拥有由NAC021基因构成的生长素信号转导靶基因蛋白互作通路。铁皮石斛和美花石斛花型差异表达miRNAs/mRNAs以及靶基因蛋白互作差异可能是导致2种石斛花型差异的主要机理。The molecular mechanism of the formation of flower diversity is still unclear.In this study,the transcriptome and microRNA sequencing of Dendrobium officinale and D.loddigesii flower organs were performed,and the miRNA-mRNA network and target protein interaction network were constructed by cytoscape and compared at the molecular level.The results of transcriptome studies showed that mRNAs related to flower development had MADS-box AP3 gene,hormone genes,etc.The miRNA-mRNA network analysis showed that the differentially expressed miRNAs and mRNAs were involved in the differential formation of floral pattern,and miR5179 and its target genes AP3,miR160and their target genes ARFs,miR164 and its target genes NAC021,miR159 and the target genes GAM1,bdi-miR159 and the target genes CKX9,miR167b-3p and the target genes ANT may be involved in floral pattern formation,and the above miRNA was upregulated in D.officinale.The protein interaction pathway showed that two kinds of dendrobium had the key gene of AP3,D.loddigesii lacked four genes:CUL1,TIR1,TIR1A and RBS;there weret wo differentially expressed ARFs genes in the auxin response interaction pathway of two Dendrobium,and D.loddigesii lacked one ARFI gene;D.loddigesii flora specifically possessed the protein interaction pathway of auxin signal transduction target genes constituted by the NAC021 gene.Differential expression of miRNAs/mRNAs in D.officinale and D.loddigesii and protein interaction of target genes may be the main mechanisms leading to the difference between the two Dendrobium flower types.

关 键 词：铁皮石斛 美花石斛 花型 转录组 small RNA 调控网络 

分 类 号：S682.31[农业科学—观赏园艺]