基于流式细胞术的兰属杂交后代倍性鉴定  

Ploidy Identification of Cymbidium Hybrid Seedlings by Flow Cytometry

作　　者：冷青云[1] 陆锦萍 黄少华[1] 徐世松[1] 李海燕[1] 牛俊海[1] 尹俊梅[1] LENG Qingyun;LU Jinping;HUANG Shaohua;XU Shisong;LI Haiyan;NIU Junhai;YIN Junmei(Tropical Crops Genetic Resources Institute,Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China,Ministry of Agriculture and Rural Affairs/Hainan Provincial Engi-neering Technology Research Center of Tropical Ornamental Plant Germplasm Innovation and Utilization/Key Laboratory of Tropical Crops Germplasm Resources Genetic Improvement and Innovation of Hainan Province,Haikou,Hainan 571101,China)

机构地区：[1]中国热带农业科学院热带作物品种资源研究所/农业农村部华南作物基因资源与种质创制重点开放实验室/海南省热带观赏植物种质创新利用工程技术研究中心/海南省热带作物资源遗传改良与创新重点实验室,海南海口571101

出　　处：《热带作物学报》2023年第11期2219-2226,共8页Chinese Journal of Tropical Crops

基　　金：国家热带植物种质资源库建设项目(No.NTPGRC2023);国家大宗蔬菜产业技术体系花卉海口综合试验站专项资金项目(No.CARS-23-G60)。

摘　　要：倍性鉴定是热带花卉种质资源鉴定及新品种选育的基础工作之一。本研究利用流式细胞仪对兰属杂交后代进行倍性鉴定。针对裂解液种类、试验材料组织器官等关键环节,以细胞核解离情况、主峰清晰程度、变异系数及取材容易程度等方面为评价指标,建立鉴定体系,并比较不同倍性植株的气孔性状差异。结果表明:CyStain UV Precise P为3种裂解液中最优,组织器官以嫩叶为宜;从3个杂交组合后代中鉴定出三倍体6株,四倍体2株,不同倍性间的气孔长度、宽度和密度均差异显著(P<0.05),气孔长度和宽度的排序为四倍体>三倍体>二倍体,而气孔密度的排序则相反。该研究结果对杂交兰多倍体选育及开展不同倍性间杂交育种具有重要应用价值。Ploidy identification is one of the basic tasks for germplasm resources and new variety breeding of tropical flowers.In this study,flow cytometry was used to identify the ploidy level of Cymbidium hybrid seedlings.aiming at the key links such as the type of dissociated liquid,the tissue organ of the test material,evaluation indexes such as the nu-clear dissociation,the clear degree of the main peak,the easy degree of sampling,and the coefficient of variation.A technique system was established and the stomatal traits of different ploidy were analyzed.The results showed that CyStain UV Precise P was the best of the three dissociated liquid and young leaves were most suitable.Six triploid and two tetraploid were identified from the offspring of the three hybrid combinations.There were significant differences in stomatal length,width and density between different ploidy(P<0.05).The rank of stomatal length and stomatal width was tetraploid>triploid>diploid,while stomatal density was reversed.This study is valuable for the polyploid selection and use of different ploidy germplasm resources for cross breeding of Cymbidium hybrid.

关 键 词：流式细胞术 兰属 倍性鉴定 

分 类 号：S682.3[农业科学—观赏园艺]