兰科蒙自兰快速繁殖和试管开花的研究  

Rapid Multiplication and in vitro Flowering of Mengzia foliosa(King & Pantl.) W.C.Huang,Z.J.Liu & C.Hu(Orchidaceae)

作　　者：邵丽[1] 曾歆花 黄卫昌[1,2] SHAO Li;ZENG Xinhua;HUANG Weichang(Shanghai Chenshan Botanical Garden/Eastern China Conservation Centre for Wild Endangered Plant Resources,Shanghai 201602,China;College of Landscape Architecture,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China)

机构地区：[1]上海辰山植物园/华东野生濒危资源植物保育中心,上海201602 [2]福建农林大学艺术园林学院,福建福州350002

出　　处：《热带作物学报》2023年第11期2236-2243,共8页Chinese Journal of Tropical Crops

基　　金：上海市科学技术委员会项目(No.19390743600);国家自然科学基金项目(No.32271740);上海市绿化和市容管理局2023年科学技术项目(No.G232408)。

摘　　要：以蒙自兰种子作为试验材料,研究不同浓度配比的生长调节剂对蒙自兰的无菌播种、快速繁殖和试管开花的影响。结果表明:人工授粉后105 d的蒙自兰种子活力强,萌发率高,1/2MS+0.6 mg/L NAA+1.0 mg/L 6-BA培养基较适合种子萌发;丛芽增殖适宜用含2.0 mL/L 6-BA的培养基,不添加NAA;生根壮苗用1/2MS+1.0 mL/L NAA+30 g/L土豆匀浆最佳;在1.0 mg/L 6-BA+0.5 mg/L NAA+1.0 mg/L PP_(333)培养基上蒙自兰开花率最高。研究结果为蒙自兰的物种保护、可持续利用以及野外种群的恢复和重建提供重要材料和技术支持。In this study,the effects of different concentration and ratio of growth regulators on seed germination,rapid propagation and in vitro flowering of Mengzia foliosa were studied.The results showed that the seeds had strong activity and high germination rate at 105 days after artificial self-pollination,and 1/2MS+0.6 mg/L NAA+1.0 mg/L 6-BA medium was more suitable for seed germination.Meanwhile,2.0 mL/L 6-BA without NAA was more suitable for cluster bud proliferation;and 1/2MS+1.0 mL/L NAA+30 g/L potato homogenate medium was the best for rooting and hardening-off.In addition,1.0 mg/L 6-BA+0.5 mg/L NAA+1.0 mg/L PP_(333) medium had the highest flowering rate.Our results not only contribute to the conservation and sustainable use of the species,but also provide important materials and technical support for wild population restoration and reconstruction.

关 键 词：蒙自兰 无菌播种 快速繁殖 试管开花 

分 类 号：S682.31[农业科学—观赏园艺]